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Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale

Prasath Paramasivam, Christian Franke, Martin Stöter, Andreas Höijer, Stefano Bartesaghi, Alan Sabirsh, Lennart Lindfors, Marianna Yanez Arteta, Anders Dahlén, Annette Bak, Shalini Andersson, Yannis Kalaidzidis, Marc Bickle, Marino Zerial

J Cell Biol (2021) 221 (2): e202110137.

DOI: https://doi.org/10.1083/jcb.202110137

Published: 09 December 2021

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Includes: Supplementary data

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Live imaging of transcription sites using an elongating RNA polymerase II–specific probe

Satoshi Uchino, Yuma Ito, Yuko Sato, Tetsuya Handa, Yasuyuki Ohkawa, Makio Tokunaga, Hiroshi Kimura

J Cell Biol (2021) 221 (2): e202104134.

DOI: https://doi.org/10.1083/jcb.202104134

Published: 02 December 2021

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Includes: Supplementary data

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Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scaling

Tobias Kletter, Sebastian Reusch, Tommaso Cavazza, Nils Dempewolf, Christian Tischer, Simone Reber

J Cell Biol (2021) 221 (1): e202106170.

DOI: https://doi.org/10.1083/jcb.202106170

Published: 17 November 2021

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High-throughput single-cell epigenomic profiling by targeted insertion of promoters (TIP-seq)

Daniel A. Bartlett, Vishnu Dileep, Tetsuya Handa, Yasuyuki Ohkawa, Hiroshi Kimura, Steven Henikoff, David M. Gilbert

J Cell Biol (2021) 220 (12): e202103078.

DOI: https://doi.org/10.1083/jcb.202103078

Published: 16 November 2021

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Includes: Supplementary data

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Rab35 controls formation of luminal projections required for bile canalicular morphogenesis

Claudiu Andrei Cozmescu, Paul Gissen

J Cell Biol (2021) 220 (10): e202108047.

DOI: https://doi.org/10.1083/jcb.202108047

Published: 13 September 2021

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Label-retention expansion microscopy

Xiaoyu Shi, Qi Li, Zhipeng Dai, Arthur A. Tran, Siyu Feng, Alejandro D. Ramirez, Zixi Lin, Xiaomeng Wang, Tracy T. Chow, Jiapei Chen, Dhivya Kumar, Andrew R. McColloch, Jeremy F. Reiter, Eric J. Huang, Ian B. Seiple, Bo Huang

J Cell Biol (2021) 220 (9): e202105067.

DOI: https://doi.org/10.1083/jcb.202105067

Published: 06 July 2021

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Includes: Supplementary data

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ESCRT, not intralumenal fragments, sorts ubiquitinated vacuole membrane proteins for degradation

Xi Yang, Lucas Reist, Dominic A. Chomchai, Liang Chen, Felichi Mae Arines, Ming Li

J Cell Biol (2021) 220 (8): e202012104.

DOI: https://doi.org/10.1083/jcb.202012104

Published: 28 May 2021

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Includes: Supplementary data

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O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport

Tae Yeon Yoo, Timothy J. Mitchison

J Cell Biol (2021) 220 (7): e202010141.

DOI: https://doi.org/10.1083/jcb.202010141

Published: 28 April 2021

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Tension promotes kinetochore–microtubule release by Aurora B kinase

Geng-Yuan Chen, Fioranna Renda, Huaiying Zhang, Alper Gokden, Daniel Z. Wu, David M. Chenoweth, Alexey Khodjakov, Michael A. Lampson

J Cell Biol (2021) 220 (6): e202007030.

DOI: https://doi.org/10.1083/jcb.202007030

Published: 27 April 2021

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Includes: Supplementary data

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Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids

John A. Smolka, Lionel A. Sanz, Stella R. Hartono, Frédéric Chédin

J Cell Biol (2021) 220 (6): e202004079.

DOI: https://doi.org/10.1083/jcb.202004079

Published: 08 April 2021

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Superresolution characterization of core centriole architecture

In Special Collection:

The Year in Cell Biology: 2021

Yuan Tian, Chenxi Wei, Jianfeng He, Yuxuan Yan, Nan Pang, Xiaomin Fang, Xin Liang, Jingyan Fu

J Cell Biol (2021) 220 (4): e202005103.

DOI: https://doi.org/10.1083/jcb.202005103

Published: 03 February 2021

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Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring

Kimberly Bellingham-Johnstun, Erica Casey Anders, John Ravi, Christina Bruinsma, Caroline Laplante

J Cell Biol (2021) 220 (3): e202008032.

DOI: https://doi.org/10.1083/jcb.202008032

Published: 26 January 2021

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Includes: Supplementary data

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Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes

Gil Kanfer, Shireen A. Sarraf, Yaakov Maman, Heather Baldwin, Eunice Dominguez-Martin, Kory R. Johnson, Michael E. Ward, Martin Kampmann, Jennifer Lippincott-Schwartz, Richard J. Youle

J Cell Biol (2021) 220 (2): e202006180.

DOI: https://doi.org/10.1083/jcb.202006180

Published: 19 January 2021

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Includes: Supplementary data

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High-content imaging-based pooled CRISPR screens in mammalian cells

Xiaowei Yan, Nico Stuurman, Susana A. Ribeiro, Marvin E. Tanenbaum, Max A. Horlbeck, Christina R. Liem, Marco Jost, Jonathan S. Weissman, Ronald D. Vale

J Cell Biol (2021) 220 (2): e202008158.

DOI: https://doi.org/10.1083/jcb.202008158

Published: 19 January 2021

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Includes: Supplementary data

Journal Articles

Easy and robust micropatterning using fibrinogen anchors

Simon Latour, Alison P. McGuigan

J Cell Biol (2021) 220 (2): e202012105.

DOI: https://doi.org/10.1083/jcb.202012105

Published: 11 January 2021

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High-efficacy subcellular micropatterning of proteins using fibrinogen anchors

Joseph L. Watson, Samya Aich, Benjamí Oller-Salvia, Andrew A. Drabek, Stephen C. Blacklow, Jason Chin, Emmanuel Derivery

J Cell Biol (2021) 220 (2): e202009063.

DOI: https://doi.org/10.1083/jcb.202009063

Published: 08 January 2021

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Journal Articles

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina

Joseph R. Tran, Danielle I. Paulson, James J. Moresco, Stephen A. Adam, John R. Yates, III, Robert D. Goldman, Yixian Zheng

J Cell Biol (2020) 220 (1): e202002129.

DOI: https://doi.org/10.1083/jcb.202002129

Published: 11 December 2020

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Includes: Supplementary data

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High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases

Ikuko Koyama-Honda, Takahiro K. Fujiwara, Rinshi S. Kasai, Kenichi G.N. Suzuki, Eriko Kajikawa, Hisae Tsuboi, Taka A. Tsunoyama, Akihiro Kusumi

J Cell Biol (2020) 219 (12): e202006125.

DOI: https://doi.org/10.1083/jcb.202006125

Published: 14 October 2020

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Includes: Supplementary data

Journal Articles

Genetically encoded live-cell sensor for tyrosinated microtubules

Shubham Kesarwani, Prakash Lama, Anchal Chandra, P. Purushotam Reddy, A.S. Jijumon, Satish Bodakuntla, Balaji M. Rao, Carsten Janke, Ranabir Das, Minhajuddin Sirajuddin

J Cell Biol (2020) 219 (10): e201912107.

DOI: https://doi.org/10.1083/jcb.201912107

Published: 04 September 2020

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Journal Articles

Object detection networks and augmented reality for cellular detection in fluorescence microscopy

Dominic Waithe, Jill M. Brown, Katharina Reglinski, Isabel Diez-Sevilla, David Roberts, Christian Eggeling

J Cell Biol (2020) 219 (10): e201903166.

DOI: https://doi.org/10.1083/jcb.201903166

Published: 26 August 2020

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Includes: Supplementary data

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NARROW

Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale

Live imaging of transcription sites using an elongating RNA polymerase II–specific probe

Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scaling

High-throughput single-cell epigenomic profiling by targeted insertion of promoters (TIP-seq)

Rab35 controls formation of luminal projections required for bile canalicular morphogenesis

Label-retention expansion microscopy

ESCRT, not intralumenal fragments, sorts ubiquitinated vacuole membrane proteins for degradation

O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport

Tension promotes kinetochore–microtubule release by Aurora B kinase

Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids

Superresolution characterization of core centriole architecture

Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring

Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes

High-content imaging-based pooled CRISPR screens in mammalian cells

Easy and robust micropatterning using fibrinogen anchors

High-efficacy subcellular micropatterning of proteins using fibrinogen anchors

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina

High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases

Genetically encoded live-cell sensor for tyrosinated microtubules

Object detection networks and augmented reality for cellular detection in fluorescence microscopy

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