Migrasomes are recently discovered vesicle-like structures on retraction fibers of migrating cells that have been linked with transfer of cellular contents, shedding of unwanted materials, and information integration. However, whether and how the cell migration paradigm regulates migrasome formation is not clear. Here, we report that there are significantly fewer migrasomes in turning cells compared with straight persistently migrating cells. The major insight underlying this observation is that as the cells elongate, their rear ends become narrower, subsequently resulting in fewer retraction fibers during impersistent migration. In addition to migration persistence, we reveal that migration speed positively corelates with migrasome formation, owing to the derived length of retraction fibers. Substantiating our hypothesis, genetically removing vimentin compromises cell migration speed and persistence and leads to fewer migrasomes. Together, our data explicate the critical roles of two cell migration patterns, persistence and speed, in the control of migrasome formation by regulating retraction fibers.
Skip Nav Destination
Article navigation
4 April 2022
Report|
February 18 2022
Cell migration orchestrates migrasome formation by shaping retraction fibers
Changyuan Fan,
Changyuan Fan
1
Unit of Cell Biology and Imaging Study of Pathogen Host Interaction, The Center for Microbes, Development and Health, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
2
University of Chinese Academy of Sciences, Beijing, China
Search for other works by this author on:
Xuemeng Shi
,
Xuemeng Shi
3
The Joint Program in Infection and Immunity, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
4
The Joint Program in Infection and Immunity, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
Search for other works by this author on:
Kaikai Zhao,
Kaikai Zhao
5
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, China
6
Biomechanics and Mechanomedicine Laboratory, Department of Engineering Mechanics, Zhejiang University, Hangzhou, China
Search for other works by this author on:
Linbo Wang,
Linbo Wang
7
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
Search for other works by this author on:
Kun Shi
,
Kun Shi
3
The Joint Program in Infection and Immunity, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
4
The Joint Program in Infection and Immunity, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
Search for other works by this author on:
Yan-Jun Liu
,
Yan-Jun Liu
8
Shanghai Institute of Cardiovascular Diseases, Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Department of Systems Biology for Medicine, Zhongshan Hospital, Fudan University, Shanghai, China
Search for other works by this author on:
Hui Li,
Hui Li
7
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
Search for other works by this author on:
Baohua Ji
,
Baohua Ji
5
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, China
6
Biomechanics and Mechanomedicine Laboratory, Department of Engineering Mechanics, Zhejiang University, Hangzhou, China
Search for other works by this author on:
Yaming Jiu
Yaming Jiu
1
Unit of Cell Biology and Imaging Study of Pathogen Host Interaction, The Center for Microbes, Development and Health, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
2
University of Chinese Academy of Sciences, Beijing, China
3
The Joint Program in Infection and Immunity, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
4
The Joint Program in Infection and Immunity, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
Search for other works by this author on:
Changyuan Fan
1
Unit of Cell Biology and Imaging Study of Pathogen Host Interaction, The Center for Microbes, Development and Health, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
2
University of Chinese Academy of Sciences, Beijing, China
Xuemeng Shi
3
The Joint Program in Infection and Immunity, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
4
The Joint Program in Infection and Immunity, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
Kaikai Zhao
5
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, China
6
Biomechanics and Mechanomedicine Laboratory, Department of Engineering Mechanics, Zhejiang University, Hangzhou, China
Linbo Wang
7
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
3
The Joint Program in Infection and Immunity, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
4
The Joint Program in Infection and Immunity, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
Yan-Jun Liu
8
Shanghai Institute of Cardiovascular Diseases, Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Department of Systems Biology for Medicine, Zhongshan Hospital, Fudan University, Shanghai, China
Hui Li
7
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
Baohua Ji
5
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, China
6
Biomechanics and Mechanomedicine Laboratory, Department of Engineering Mechanics, Zhejiang University, Hangzhou, China
Yaming Jiu
1
Unit of Cell Biology and Imaging Study of Pathogen Host Interaction, The Center for Microbes, Development and Health, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
2
University of Chinese Academy of Sciences, Beijing, China
3
The Joint Program in Infection and Immunity, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China
4
The Joint Program in Infection and Immunity, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
Correspondence to Yaming Jiu: ymjiu@ips.ac.cn
Received:
October 05 2021
Revision Received:
January 05 2022
Accepted:
January 23 2022
Online Issn: 1540-8140
Print Issn: 0021-9525
Funding
Funder(s):
National Natural Science Foundation of China
- Award Id(s): 2054104,31970660
Funder(s):
CAS-VPST Silk Road Science
- Award Id(s): GJHZ2021138
Funder(s):
Ministry of Science and Technology of China
- Award Id(s): 2021YFC2300204
Funder(s):
Shanghai Municipal Science and Technology
- Award Id(s): 2019SHZDZX02
Funder(s):
Institut Pasteur of Shanghai
- Award Id(s): KLMVI-OP-202001
This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
2022
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2022) 221 (4): e202109168.
Article history
Received:
October 05 2021
Revision Received:
January 05 2022
Accepted:
January 23 2022
Citation
Changyuan Fan, Xuemeng Shi, Kaikai Zhao, Linbo Wang, Kun Shi, Yan-Jun Liu, Hui Li, Baohua Ji, Yaming Jiu; Cell migration orchestrates migrasome formation by shaping retraction fibers. J Cell Biol 4 April 2022; 221 (4): e202109168. doi: https://doi.org/10.1083/jcb.202109168
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your Institution
2,054
Views
0
Citations
Advertisement
Advertisement