The plasma membrane fraction of rat liver was isolated and incubated with labeled lysophosphatides in the presence of cofactors; the acylation of lysolecithin to lecithin by the fraction was compared to that of the rough and smooth microsomes. The purity of the isolated fractions was ascertained by enzyme markers and electron microscopy, and the maximal contamination of the plasma membrane fraction by microsomes did not exceed 20%. Under conditions at which the reaction was proportional to the amount of enzyme used, the plasma membrane had a specific activity similar to that of the smooth and rough microsomes. With doubly labeled lysolecithin (containing palmitic acid-14C and choline-3H) it was shown that the lecithin formed retained the same ratio of the two labels, which indicated that lysolecithin was converted to lecithin through an acylation reaction. The newly formed lecithin was shown to be bound to the plasma membrane fraction; this suggested that it is incorporated into the structure of the membrane itself.
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October 01 1968
ACYLATION OF LYSOPHOSPHATIDES BY PLASMA MEMBRANE FRACTIONS OF RAT LIVER
Yechezkiel Stein,
Yechezkiel Stein
From The Rockefeller University, New York, New York 10021.
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Christopher Widnell,
Christopher Widnell
From The Rockefeller University, New York, New York 10021.
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Olga Stein
Olga Stein
From The Rockefeller University, New York, New York 10021.
Search for other works by this author on:
Yechezkiel Stein
From The Rockefeller University, New York, New York 10021.
Christopher Widnell
From The Rockefeller University, New York, New York 10021.
Olga Stein
From The Rockefeller University, New York, New York 10021.
Dr. Stein's present address is Laboratory of Lipid Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel
Received:
May 10 1968
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1968 by The Rockefeller University Press.
1968
J Cell Biol (1968) 39 (1): 185–192.
Article history
Received:
May 10 1968
Citation
Yechezkiel Stein, Christopher Widnell, Olga Stein; ACYLATION OF LYSOPHOSPHATIDES BY PLASMA MEMBRANE FRACTIONS OF RAT LIVER . J Cell Biol 1 October 1968; 39 (1): 185–192. doi: https://doi.org/10.1083/jcb.39.1.185
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