Filamentous actin in living cultured cells was labeled by microinjecting trace amounts of rhodamine-phalloidin (rh-pha) as a specific, high-affinity probe. The microinjection caused no detectable effect on cell morphology or cell division. The distribution of rh-pha-labeled filaments was then examined in dividing cells using image-intensified fluorescence microscopy, and the exchangeability of labeled filaments along stress fibers was studied during interphase using fluorescence recovery after photobleaching. rh-pha showed a rapid concentration at the contractile ring during cell division. In addition, recovery of fluorescence after photobleaching occurred along stress fibers with a halftime as short as 8 min. These observations suggest that at least some actin filaments undergo continuous movement and reorganization in living cells. This dynamic process may play an important role in various cellular functions.
Article| December 01 1987
Mobility of filamentous actin in living cytoplasm.
Y L Wang
Doris W. Neustadt Laboratory of Cellular Structure, Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1987) 105 (6): 2811–2816.
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Y L Wang; Mobility of filamentous actin in living cytoplasm.. J Cell Biol 1 December 1987; 105 (6): 2811–2816. doi: https://doi.org/10.1083/jcb.105.6.2811
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