We have investigated the sorting and packaging of secretory proteins into secretory granules by an immunological approach. An mAb against secretogranin I (chromogranin B), a secretory protein costored with various peptide hormones and neuropeptides in secretory granules of many endocrine cells and neurons, was expressed by microinjection of its mRNA into the secretogranin I-producing cell line PC12. An mAb against the G protein of vesicular stomatitis virus--i.e., against an antigen not present in PC12 cells--was expressed as a control. The intracellular localization and the secretion of the antibodies was studied by double-labeling immunofluorescence using the conventional and the confocal microscope, as well as by pulse-chase experiments. The secretogranin I antibody, like the control antibody, was transported along the secretory pathway to the Golgi complex. However, in contrast to the control antibody, which was secreted via the constitutive pathway, the secretogranin I antibody formed an immunocomplex with secretogranin I, was packaged into secretory granules, and was released by regulated exocytosis. Our results show that a constitutive secretory protein, unaltered by genetic engineering, can be diverted to the regulated pathway of secretion by its protein-protein interaction with a regulated secretory protein. The data also provide the basis for immunologically studying the role of luminally exposed protein domains in the biogenesis and function of regulated secretory vesicles.
Article|
July 01 1989
An antibody against secretogranin I (chromogranin B) is packaged into secretory granules.
P Rosa,
P Rosa
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Search for other works by this author on:
U Weiss,
U Weiss
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Search for other works by this author on:
R Pepperkok,
R Pepperkok
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Search for other works by this author on:
W Ansorge,
W Ansorge
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Search for other works by this author on:
C Niehrs,
C Niehrs
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Search for other works by this author on:
E H Stelzer,
E H Stelzer
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Search for other works by this author on:
W B Huttner
W B Huttner
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Search for other works by this author on:
P Rosa
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
U Weiss
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
R Pepperkok
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
W Ansorge
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
C Niehrs
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
E H Stelzer
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
W B Huttner
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1989) 109 (1): 17–34.
Citation
P Rosa, U Weiss, R Pepperkok, W Ansorge, C Niehrs, E H Stelzer, W B Huttner; An antibody against secretogranin I (chromogranin B) is packaged into secretory granules.. J Cell Biol 1 July 1989; 109 (1): 17–34. doi: https://doi.org/10.1083/jcb.109.1.17
Download citation file: