Bradyrhizobium japonicum (R110d) exhibited four saccharide-specific binding activities: (a) adsorption to Sepharose beads containing covalently coupled lactose; (b) homotypic agglutination through one pole of the cell (star formation); (c) heterotypic adhesion to the cultured soybean cell line, SB-1; and (d) attachment to roots of soybean plants. Each of these binding activities can be inhibited by the addition of galactose or lactose, but not by derivatives such as N-acetyl-D-galactosamine or melibiose. Treatment of wild-type bacteria with N-methyl-N'-nitro-N-nitrosoguanidine followed by selection on the basis of reduced binding to SB-1 cells, resulted in two specific mutants, designated N4 and N6. Compared to wild type, these two mutants also exhibited decreased binding activity in: (a) adsorption to lactose-Sepharose beads; (b) homotypic star formation; and (c) heterotypic attachment to roots of soybeans plants. These results suggest that all four of the saccharide-inhibitable binding activities of Bradyrhizobium japonicum may be mediated by the same mechanism(s) or molecular component(s).
Article|
October 01 1990
Carbohydrate binding activities of Bradyrhizobium japonicum. I. Saccharide-specific inhibition of homotypic and heterotypic adhesion.
S C Ho,
S C Ho
Department of Biochemistry, Michigan State University, East Lansing 48824.
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J L Wang,
J L Wang
Department of Biochemistry, Michigan State University, East Lansing 48824.
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M Schindler
M Schindler
Department of Biochemistry, Michigan State University, East Lansing 48824.
Search for other works by this author on:
S C Ho
Department of Biochemistry, Michigan State University, East Lansing 48824.
J L Wang
Department of Biochemistry, Michigan State University, East Lansing 48824.
M Schindler
Department of Biochemistry, Michigan State University, East Lansing 48824.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1990) 111 (4): 1631–1638.
Citation
S C Ho, J L Wang, M Schindler; Carbohydrate binding activities of Bradyrhizobium japonicum. I. Saccharide-specific inhibition of homotypic and heterotypic adhesion.. J Cell Biol 1 October 1990; 111 (4): 1631–1638. doi: https://doi.org/10.1083/jcb.111.4.1631
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