The addition of exogenous hepatocyte growth factor (HGF)/scatter factor (SF) to MDCK epithelial cells results in fibroblastic morphology and cell motility. We generated HGF/SF producing MDCK cells by transfection with an expression plasmid containing human HGF/SF cDNA. Production of HGF/SF by these cells induced a change from an epithelial to a fibroblastic morphology and increased cell motility. In addition, the HGF/SF producing cells acquired efficient anchorage-independent growth in soft agar but did not form tumors in nude mice. The morphological change and the stimulation of the anchorage-independent growth were prevented by anti-HGF/SF antibody, suggesting that the factor is secreted and then exerts its effects through cell surface receptors.
Article|
May 15 1992
Expression of a human hepatocyte growth factor/scatter factor cDNA in MDCK epithelial cells influences cell morphology, motility, and anchorage-independent growth
Y Uehara,
Y Uehara
Institute for Liver Research, Kansai Medical University, Osaka, Japan.
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N Kitamura
N Kitamura
Institute for Liver Research, Kansai Medical University, Osaka, Japan.
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Y Uehara
Institute for Liver Research, Kansai Medical University, Osaka, Japan.
N Kitamura
Institute for Liver Research, Kansai Medical University, Osaka, Japan.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1992) 117 (4): 889–894.
Citation
Y Uehara, N Kitamura; Expression of a human hepatocyte growth factor/scatter factor cDNA in MDCK epithelial cells influences cell morphology, motility, and anchorage-independent growth. J Cell Biol 15 May 1992; 117 (4): 889–894. doi: https://doi.org/10.1083/jcb.117.4.889
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