E-selectin elicits cell adhesion by binding to the cell surface carbohydrate, sialyl Lewis X (sLe(x)). We evaluated the effects of mutations in the E-selectin lectin domain on the binding of a panel of anti-E-selectin mAbs and on the recognition of immobilized sLe(x) glycolipid. Functional residues were then superimposed onto a three-dimensional model of the E-selectin lectin domain. This analysis demonstrated that the epitopes recognized by blocking mAbs map to a patch near the antiparallel beta sheet derived from the NH2 and COOH termini of the lectin domain and two adjacent loops. Mutations that affect sLe(x) binding map to this same region. These results thus define a small region of the E-selectin lectin domain that is critical for carbohydrate recognition.
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October 01 1992
Identification of an E-selectin region critical for carbohydrate recognition and cell adhesion.
D V Erbe,
D V Erbe
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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B A Wolitzky,
B A Wolitzky
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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L G Presta,
L G Presta
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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C R Norton,
C R Norton
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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R J Ramos,
R J Ramos
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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D K Burns,
D K Burns
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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J M Rumberger,
J M Rumberger
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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B N Rao,
B N Rao
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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C Foxall,
C Foxall
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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B K Brandley
B K Brandley
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
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D V Erbe
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
B A Wolitzky
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
L G Presta
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
C R Norton
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
R J Ramos
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
D K Burns
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
J M Rumberger
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
B N Rao
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
C Foxall
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
B K Brandley
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1992) 119 (1): 215–227.
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D V Erbe, B A Wolitzky, L G Presta, C R Norton, R J Ramos, D K Burns, J M Rumberger, B N Rao, C Foxall, B K Brandley; Identification of an E-selectin region critical for carbohydrate recognition and cell adhesion.. J Cell Biol 1 October 1992; 119 (1): 215–227. doi: https://doi.org/10.1083/jcb.119.1.215
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