Neuronal growth factors regulate the expression of voltage-activated sodium current in differentiating sympathetic neurons and PC12 cells. We show that, in PC12 cells, the NGF- and FGF-induced sodium current results from increased expression of two distinct sodium channel types. Sodium current results from the rapid induction of a novel sodium channel transcript, also found in peripheral neurons, and from the long term induction of brain type II/IIA mRNA. Expression of the type II/IIA sodium channel requires activation of the cyclic AMP-dependent protein kinase (A-kinase), whereas induction of the peripheral neuron type sodium channel occurs through an A-kinase-independent signal transduction pathway. These findings suggest that the two sodium channel types act in concert to ensure the generation of action potentials during neuronal differentiation.
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August 15 1993
Neuronal growth factor regulation of two different sodium channel types through distinct signal transduction pathways
G D'Arcangelo,
G D'Arcangelo
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
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K Paradiso,
K Paradiso
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
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D Shepherd,
D Shepherd
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
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P Brehm,
P Brehm
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
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S Halegoua,
S Halegoua
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
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G Mandel
G Mandel
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
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G D'Arcangelo
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
K Paradiso
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
D Shepherd
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
P Brehm
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
S Halegoua
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
G Mandel
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1993) 122 (4): 915–921.
Citation
G D'Arcangelo, K Paradiso, D Shepherd, P Brehm, S Halegoua, G Mandel; Neuronal growth factor regulation of two different sodium channel types through distinct signal transduction pathways. J Cell Biol 15 August 1993; 122 (4): 915–921. doi: https://doi.org/10.1083/jcb.122.4.915
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