Direct Involvement of Ezrin/Radixin/Moesin (ERM)-binding Membrane Proteins in the Organization of Microvilli in Collaboration with Activated ERM Proteins

CV-1 monkey kidney cells (Jensen et al., 1964) and A431 human epidermoid cells (Giard et al., 1973) were obtained from Health Science Research Resources Bank. Mouse fibroblastic L cells (Earle, 1943) and MTD-1A epithelial cells (Enami et al., 1984) were provided by M. Takeichi (Kyoto University, Kyoto, Japan). These cells were cultured in DME supplemented with 10% FCS.

Mouse anti-ERM mAb (CR22) with higher affinity to moesin than to ezrin/radixin (Sato et al., 1991), rat anti-ezrin mAb (M11), rat anti-radixin mAb (R2-1) and rat anti-moesin mAb (M22) (Takeuchi et al., 1994), rat anti–COOH-terminal phosphorylated ERM (CPERM) mAb (297S) specific for phosphorylated ERM proteins at T567, T564, or T558 for ezrin, radixin, or moesin, respectively (Matsui et al., 1998), and rabbit anti-ERM polyclonal antibodies (pAbs) (TK88 and TK89) (Kondo et al., 1997) were used. TK88 was raised in a rabbit against synthesized peptides corresponding to the mouse ERM proteins' common sequence (293–302) and detected all ERM proteins. Rabbit anti–E-cadherin pAb (Nagafuchi et al., 1987) and rat anti–E-cadherin mAb (ECCD-2; Shirayoshi et al., 1986) were provided by M. Takeichi. IM7.8.1 is a rat anti–mouse CD44 mAb (Trowbridge et al., 1982). Mouse anti–phosphotyrosine mAb (clone 4G10) and mouse anti-vesicular stomatitis virus glycoprotein G (VSVG) mAb (clone P5D4) were purchased from Upstate Biotechnology Inc. and Sigma Chemical Co., respectively.

Some vectors used in this study were described previously (Yonemura et al., 1993, 1998). E-43, E-44, or E-ICAM-2 represents a chimeric molecule consisting of the extracellular domain of mouse E-cadherin and transmembrane/cytoplasmic domain of rat CD43, mouse CD44, or mouse ICAM-2, respectively. The extracellular domain of E-cadherin was used as an epitope tag to detect the cell surface expression of each chimeric molecule in L fibroblasts expressing no detectable E-cadherin. Several mutant proteins, E-43/1-31, E-44/1-19, E-44/20-70, and E-43/KRR:NGG, were described in detail previously (Yonemura et al., 1998). To obtain cDNA encoding VSVG-tagged ezrin (pSK/mEz-VSVG), an oligonucleotide encoding 11 COOH-terminal amino acids of VSVG was ligated into the 3′ terminal end of mouse ezrin cDNA (Funayama et al., 1991) according to Algrain et al. (1993). A cDNA fragment of pSK/mEz-VSVG was inserted into the HindIII-HpaI fragment of the pβact-CAT9 (Fregien and Davidson, 1986) to construct its expression vector (pA/mEz-VSVG). Plasmids encoding ezrin mutants on the COOH-terminal threonine (T567) were generated by PCR with appropriate primers from pSK/mEz-VSVG. Alanine or aspartic acid was substituted for T567 (pA/mEz-T/A-VSVG or pA/mEz-T/D-VSVG, respectively).

Cells were transfected with DNA using Lipofectin or Lipofectamine (GIBCO BRL). Cultured cells on coverslips were washed twice with Opti-MEM (GIBCO BRL), and incubated for 3–5 h with 1 ml Opti-MEM containing 1 μg of plasmid DNAs and 10 μl of the reagents, followed by addition of 3 ml of normal medium containing FCS. Cells were then cultured for 3–5 d. Since transfection with Lipofectin or Lipofectamine occasionally caused damage to the cell surface structures of transfected cells, cells were also transfected by microinjection using a set of manipulators (MN-188 and MO-189; Narishige) connected to an Eppendorf microinjector 5242 (Eppendorf, Inc.). Expression vectors in injection buffer (100 mM KCl, 10 mM Hepes buffer, pH 7.5) were injected into the nuclei of cells cultured on coverslips. Cells were examined 12–24 h after injection.

Subconfluent A431 cells on coverslips in DME with 10% FCS were transferred to DMEM without FCS and cultured for 6–12 h. Recombinant human EGF (GIBCO BRL) was added at a final concentration of 100 ng/ml. Cells were incubated for 30 s, and quickly fixed and processed for immunofluorescence microscopy or SDS-PAGE followed by immunoblotting. In some experiments, cells were microinjected with expression vectors, allowed to express proteins for 6–18 h, serum-starved, treated with EGF, and then processed for immunofluorescence microscopy.

Cells were fixed with 1–4% fresh formaldehyde in 0.1 M Hepes buffer (pH 7.5) for 15 min. After three washes with PBS containing 30 mM glycine (G-PBS), cells were soaked in blocking solution (G-PBS containing 2% normal goat serum) for 5 min and incubated with anti– E-cadherin mAb (ECCD-2) diluted with the blocking solution for 10 min. Cells were then washed three times with G-PBS, treated with 0.2% Triton X-100 in G-PBS for 10 min, and washed with G-PBS. Cells were then soaked in blocking solution for 10 min, incubated with CR22 for 30 min, washed three times with G-PBS, and incubated with secondary antibodies for 30 min. DTAF-conjugated goat anti–rat IgG antibody (Chemicon) and Cy3-conjugated goat anti–mouse IgG antibody (Amersham International) were used as secondary antibodies. For triple labeling, FITC-phalloidin (Molecular Probes, Inc.), Cy3-conjugated goat anti–rat IgG antibody (Amersham International), and Cy5-conjugated goat anti–mouse IgG antibody (Amersham International) were used. Cells were washed three times, then mounted in 90% glycerol-PBS containing 0.1% para-phenylenediamine and 1% n-propylgalate. Specimens were observed using a Zeiss Axiophot photomicroscope (Carl Zeiss) with appropriate combinations of filters and mirrors. Photographs were taken on T-MAX 400 film (Eastman Kodak Co.), or recorded with a cooled CCD camera (SenSys 0400, 768 × 512 pixels; Photometrics) controlled by a Power Macintosh 7600/132 and the software package IPLab Spectrum V3.1 (Signal Analytics Corp.).

Cells were fixed and doubly stained with anti–E-cadherin mAb (ECCD-2) and anti-ERM mAb (CR22) as described above. Alternatively, cells were microinjected with expression vectors, washed with cold culture medium the next day, and incubated with cold hybridoma culture medium containing anti-CD44 mAb (IM7.8.1) and anti– E-cadherin pAb for 15 min at 4°C. After washing twice with cold culture medium, cells were fixed with 1% formaldehyde in 0.1 M Hepes buffer (pH 7.5) for 15 min at room temperature. Cells were washed, permeabilized, and soaked in blocking solution. Cy2-conjugated goat anti–rabbit IgG antibody (Amersham International) and Cy3-conjugated goat anti– rat IgG antibody were used as secondary antibodies.

For double labeling of cells with 297S and TK88 to detect CPERMs and total ERM proteins, respectively, cells were fixed with 10% trichloroacetic acid for 15 min on ice (Hayashi et al., 1999). In this case, TK89 which also detected all ERM proteins was not used, since the epitope for 297S is overlapped with that for TK89. To label cells with ECCD-2, TK89, P5D4, and/or FITC-phalloidin, they were fixed with 4% formaldehyde in 0.1 M Hepes buffer (pH 7.5) for 15 min. Cells were washed, permeabilized, and soaked in blocking solution. Cy2-conjugated goat anti–rabbit IgG antibody, Cy2-conjugated donkey anti–mouse IgG antibody (Jackson ImmunoResearch Laboratories, Inc.), Cy3-conjugated goat anti–mouse IgG (Amersham), Cy3-conjugated goat anti–rat IgG antibody, and/or Cy5-conjugated goat anti-mouse IgG antibody were used as secondary antibodies. In A431 cells, cell surface expression of E-cadherin-chimeric molecules was hard to detect because these cells express considerable amounts of endogenous E-cadherin. However, E-cadherin-chimeric molecules could be detected by staining cells with ECCD-2 after permeabilization with Triton X-100 because these molecules, but not endogenous E-cadherin, were accumulated in the cytoplasm in large amounts.

Polystyrene latex beads with a sulfate group on their surface (0.1 μm FluoSpheres; Molecular Probes) were coated with avidin according to the method of Molday et al. (1974). 1 d after microinjection with the expression vector encoding E-43, CV-1 cells on coverslips were fixed with 4% formaldehyde in 0.1 M Hepes buffer (pH 7.5) for 20 min, washed with G-PBS three times, soaked in blocking solution for 10 min, and then incubated in blocking solution containing anti–E-cadherin mAb, ECCD-2 for 20 min. Cells were washed with G-PBS, incubated with biotinylated goat anti–rat IgG antibody (Amersham International) in blocking solution, and then washed with G-PBS. Samples were incubated with the avidin-coated beads suspended in blocking solution for 1 h. Cells were washed with G-PBS and fixed with 2.5% glutaraldehyde in 0.1 M Hepes buffer (pH 7.5) for 2 h followed by postfixation with the same buffer containing 1% OsO₄ at 4°C for 1 h. Samples were then washed with distilled water, dehydrated through a graded series of ethanol, transferred into isoamyl acetate, and dried in a critical point drier (Hitachi Co.) after substitution with liquid CO₂. Dried samples were coated with gold using a gold sputter coater (Eiko Engineering), and were examined under a scanning electron microscope (model S-800; Hitachi Co.).

Subconfluent A431 cells cultured on a 35-mm dish were serum-starved for 6 h as described above. With or without EGF treatment (100 ng/ml) for 30 s, cells were washed with ice-cold PBS twice, and then recovered in 200 μl of SDS-PAGE sample buffer by scraping. Proteins in 20 μl of the sample were separated by SDS-PAGE and transferred onto nitrocellulose membranes. Blots were first incubated with anti-phosphotyrosine mAb (4G10), which were localized using the enhanced chemiluminescence Western blotting detection system (Amersham). Bound antibodies were then removed from the membranes by incubating with 7 M guanidine-HCl, 50 μM EDTA, 20 mM 2-mercaptoethanol for 10 min at room temperature. These membranes were incubated with anti-CPERM mAb (297S) to detect threonine-phosphorylated ERM proteins, then bound mAb was again removed. Finally, these membranes were incubated with anti-ERM protein pAb (TK89) to total ERM proteins.

In our previous study, we transfected L cells with cDNAs encoding several chimeric molecules containing the extracellular domain of E-cadherin and the transmembrane/cytoplasmic domain of CD43, CD44, or ICAM-2 (E-43, E-44, or E-ICAM-2, respectively), and showed that these chimeric molecules were concentrated in microvilli together with ERM proteins (Yonemura et al., 1998). Then, we attempted to examine the abilities of these chimeric molecules to elongate microvilli using stable transfectants. However, the microvillar elongation was not clearly observed in these cells, probably because the stable transfectants expressed relatively low levels of chimeric molecules. Next, we examined the effects of transient overexpression of these chimeric molecules on L cell surface morphology.

The expression and localization of each chimeric molecule was detected with anti–E-cadherin mAb, ECCD-2, which recognized the extracellular domain of E-cadherin, and microvilli were specifically visualized by immunofluorescence staining with anti-ERM protein mAb, CR22. As shown in Fig. 1, a and b, when the expression level of E-43 was sufficiently high, expressed E-43 was not only colocalized with ERM proteins in microvilli but also caused elongation of microvilli, as compared to nontransfected L cells (Fig. 1 g). The number of microvilli did not appear to increase. Overexpression of E-44 or E-ICAM-2 gave the same results (Fig. 1, c–f). Overexpression of E-43/1-31 (a deletion mutant of E-43 lacking the COOH-terminal region but which carries the juxta-membrane ERM-binding region of CD43 cytoplasmic domain; see Yonemura et al., 1998) also induced microvillar elongation (data not shown). Immunofluorescence microscopy with ezrin-, radixin-, or moesin-specific mAbs revealed that in all cases ezrin, radixin, and moesin were recruited to the elongated microvilli without any bias (data not shown).

Two distinct control experiments were performed. First, wild-type E-cadherin was introduced into L cells. As previously reported (Yonemura et al., 1998), E-cadherin was distributed diffusely over the cell surface (Fig. 2 a), and the length and number of ERM-positive microvilli were not changed (Fig. 2 b). Second, two chimeric molecules lacking the ERM-binding ability were introduced into L cells: E-44/20-70 in which the juxta-membrane ERM-binding region was deleted from the E-44 cytoplasmic domain and E-43/KRR:NGG in which the juxta-membrane ERM-binding region of E-43 was disrupted by site-directed mutation (see Yonemura et al., 1998). These chimeric molecules did not cause elongation of microvilli in transient transfectants (Fig. 2, c–f).

Furthermore, the effects of overexpression of E-43 on the cortical actin cytoskeleton organization were examined (Fig. 3). In the dorsal cortex of nontransfected L cells, stress fibers and relatively short microvillar core actin bundles were observed (arrow in Fig. 3 b). In the dorsal cortex of E-43–expressing cells, stress fibers were disrupted with concomitant formation of relatively long microvillar actin bundles (asterisk in Fig. 3 b), whereas ventral stress fibers were not affected (data not shown). In E-cadherin–overexpressing cells, dorsal stress fibers were not affected (data not shown).

Next, another fibroblastic cell line, CV-1, was used. CV-1 cells bear short microvilli and were used to examine the effects of villin on microvillar elongation (Friederich et al., 1989). When E-43 was transiently introduced into CV-1 cells, microvillar elongation was more clearly demonstrated than in L cells (Fig. 4). The length of microvilli was then quantitatively compared between nontransfected and E-43–expressing CV-1 cells using anti–ERM mAb-stained images (Fig. 5). In nontransfected cells, microvilli were mostly shorter than 1 μm (0.53 ± 0.33 μm; n = 320), whereas in E-43–expressing cells most microvilli were 3–4 μm in length (3.5 ± 1.4 μm; n = 300). These results are comparable with those in villin-induced microvilli (2.4 μm) (Friederich et al., 1989). Finally, the surface morphology of these cells was examined by immunoscanning electron microscopy (Fig. 6). To detect cells transiently expressing E-43, the extracellular domain of E-cadherin was detected with anti–E-cadherin mAb labeled with biotinylated secondary antibody followed by avidin-conjugated beads. The surface of nontransfected cells lacking bead labeling was characterized by numerous short microvilli (Fig. 6 a). In contrast, many elongated microvilli were observed on the surface of E-43–expressing cells together with scattered beads (Fig. 6 b).

When E-43 was introduced into cultured MTD-1A epithelial cells, E-43 was concentrated at ERM-positive microvilli at their apical membrane surface, but did not induce apparent elongation of microvilli (Fig. 7, a and b). Instead, in these E-43–overexpressing cells, the endogenous microvillar integral membrane protein, CD44, was significantly excluded from microvilli (Fig. 7, c and d). Overexpression of E-44 also gave the same results, whereas that of E-cadherin did not affect the microvillar localization of endogenous CD44 (Fig. 7, e–h). This effect appeared to be dependent on the level of E-43 or E-44 expression. In cultured fibroblastic L cells, E-43, E-44, or E-ICAM-2 did not cause exclusion of endogenous CD44 from microvilli (data not shown).

Another epithelial cell line, A431, was examined. Similar to MTD-1A cells, overexpression of E-43 (Fig. 8, a and b), E-44, or E-ICAM-2 (data not shown) did not induce elongation of microvilli. In A431 cells, EGF treatment was reported to induce morphological changes in the cell surface such as microvillar elongation and ruffling with concomitant activation of ezrin (Chinkers et al., 1979; Bretscher, 1989; Franck et al., 1993; Berryman et al., 1995). Interestingly, as shown in Fig. 8, c and d, when E-43–overexpressing cells were treated with EGF, within 30 s significantly longer microvilli appeared on the cell surface as compared to surrounding nontransfected cells. Ezrin was clearly recruited into and concentrated in these overelongated microvilli. This type of EGF-dependent overelongation of microvilli was not observed in A431 cells expressing E-43/KRR:NGG that has no binding ability to ERM proteins (Fig. 8, e and f).

The difference in the effects of overexpression of ERMBMPs between fibroblastic and epithelial cells suggested that in fibroblasts ERM proteins are more efficiently activated than in epithelial cells (see Fig. 11). If this is the case, it is likely that in A431 cells EGF effectively activates ERM proteins. ERM proteins phosphorylated at the COOH-terminal threonine residue (T567, T564, and T558 in ezrin, radixin, and moesin, respectively) have been proposed to function as active forms in cross-linking ERMBMPs to actin filaments (Nakamura et al., 1995; Matsui et al., 1998; Oshiro et al., 1998; Pietromonaco et al., 1998; Shaw et al., 1998; Simons et al., 1998; Hayashi et al., 1999). We examined the effects of EGF on the threonine phosphorylation of ERM proteins in A431 cells (Fig. 9).

As reported previously (Hunter and Cooper, 1981; Bretscher, 1989; Franck et al., 1993), immunoblotting of EGF-treated A431 cells with anti-phosphotyrosine mAb revealed that ezrin as well as EGF receptor were heavily tyrosine-phosphorylated (Fig. 9 A, upper panel). In A431 cells with or without EGF treatment, anti-ERM pAb (TK89) identified almost equal amounts of ezrin and moesin, but only a trace amount of radixin (Fig. 9 A, lower panel). Previously, we generated mAb 297S which specifically recognized CPERMs (Matsui et al., 1998). Immunoblotting with this mAb clearly revealed that the amount of CPERMs was increased in A431 cells after EGF treatment (Fig. 9 A, middle panel). Importantly, not only ezrin but also moesin were phosphorylated at their COOH-terminal threonine residues. MTD-1A cells and L cells contained CPERMs in comparable amounts to EGF-treated A431 cells (Fig. 9 A).

We then compared the behavior of CPERMs with that of total ERM proteins by immunofluorescence microscopy during the activation of ERM proteins induced by EGF treatment (Fig. 9 B). Before EGF treatment, CPERMs were hardly detected with some exceptional signals from the cell surface (Fig. 9 B, panel a). Total ERM proteins were distributed diffusely in the cytoplasm and in a punctate manner on the dorsal surface (Fig. 9 B, panel b). When cells were treated with EGF for 30 s, the staining intensity of CPERMs was increased markedly on cell surface structures including elongating microvilli but not in the cytoplasm (Fig. 9 B, panel c). In contrast, the total ERM proteins were still detected in large amounts in the cytoplasm in addition to the cell surface (Fig. 9 B, panel d). These observations favored the suggestion that CPERMs represent activated ERM proteins.

To further evaluate this suggestion experimentally, we performed site-directed mutagenesis on ezrin which was predominant among ERM proteins in A431 cells. The COOH-terminally VSVG-tagged ezrin (Ez-VSVG) was shown previously to behave similarly to endogenous ezrin (Algrain et al., 1993; Crepaldi et al., 1997). Also, in serum-starved A431 cells Ez-VSVG was codistributed with endogenous ERM proteins (Fig. 10, a and b). When the COOH-terminal threonine residue of ezrin (T567) was mutated to alanine (Ez-T/A-VSVG; nonphosphorylatable ezrin), the expressed mutant ezrin was again codistributed with endogenous ezrin in the cytoplasm as well as on the cell surface of serum-starved A431 cells (Fig. 10, c and d). In sharp contrast, when T567 was mutated to aspartic acid (Ez-T/D-VSVG), which was expected to mimic the T567-phosphorylated form of ezrin, the expressed mutant was preferably enriched at the cell surface to elongate microvilli in serum-starved A431 cells (Fig. 10 e). These elongated microvilli contained actin filaments along their length (Fig. 10, g and h), and the expressed Ez-T/D-VSVG often formed large aggregates on the cell surface or in the cytoplasm where actin filaments were recruited (arrows in Fig. 10, g and h). In contrast, when Ez-T/D-VSVG was introduced into L cells, no elongation of microvilli was detected (data not shown).

Although microvilli are ubiquitous membrane surface structures, their physiological functions and the molecular mechanism behind their organization have not been fully elucidated. It has been reported that overexpression of some cytoskeletal or lipid-binding proteins results in the elongation of microvilli (Friederich et al., 1989; Arpin et al., 1994b; Ma et al., 1997), but no information regarding the involvement of integral membrane proteins in the microvillar organization is available. In this study, we first showed that overexpression of several ERMBMPs such as E-43, E-44, and E-ICAM-2 induced the elongation of microvilli in fibroblasts such as L and CV-1 cells. This activity of these integral membrane proteins was dependent on their direct binding to ERM proteins, since the removal of the ERM-binding domains from their cytoplasmic domains resulted in loss of activity. Furthermore, as shown in Fig. 3, this ERMBMP-dependent elongation of microvilli was associated with disruption of the dorsal stress fibers, suggesting that the expressed ERMBMPs recruited not only ERM proteins but also actin filaments. Of course, the possibility cannot be excluded that unidentified cytoplasmic proteins other than ERM proteins are also involved in such integral membrane protein-induced microvillar organization in some types of cells. In human RPM-MC melanoma cells, CD44 mutants lacking the ezrin-binding domain were reported to still localize at microvilli together with ezrin (Legg and Isacke, 1998), and in some types of tissues CD44 and ERM proteins are not necessarily colocalized (Berryman et al., 1995; von Andrian et al., 1995; Nakamura and Ozawa, 1996).

Interestingly, in epithelial cells such as MTD-1A and A431 cells, these ERMBMPs did not induce the elongation of microvilli. Instead, they caused exclusion of endogenous CD44 from preexisting microvilli in MTD-1A cells. This differential behavior of ERMBMPs in epithelial and fibroblastic cells could be explained if the pool size of activated ERM proteins is larger in fibroblastic cells than in epithelial cells. However, immunoblotting did not identify any significant difference in the amount of CPERMs (which may correspond to activated ERM proteins as discussed below) between fibroblastic L cells and epithelial MTD-1A cells (Fig. 9 A). Thus, as shown schematically in Fig. 11, A and B, we postulated that in epithelial cells used in this study the ability to convert ERM proteins from inactive form to active form was suppressed as compared to fibroblastic cells. In response to increases in the amount of ERMBMPs, ERM proteins in the cytoplasm of fibroblasts would be efficiently activated with their concomitant recruitment to organize microvilli. In contrast, since in epithelial cells the activation of ERM proteins in response to increases in the amount of ERMBMPs was suppressed, the introduced ERMBMPs would compete out endogenous CD44 from the membrane-bound activated ERM proteins in preexisting microvilli. This interpretation was supported by the observations in EGF-treated A431 cells (Fig. 11, C–E). Bretscher and colleagues reported that EGF treatment increased the levels of tyrosine phosphorylation and oligomerization of ezrin in A431 cells, resulting in the elongation of microvilli and membrane ruffles by recruiting ezrin (Bretscher, 1989; Berryman et al., 1995). These findings suggest that in these cells, EGF directly or indirectly released the suppression of the ezrin (and probably ERM protein) activation process (Fig. 11 C). The present results indicated that in these EGF-treated A431 cells, in which ERM proteins could be efficiently activated in response to increases in the amount of ERMBMPs, the overexpression of E-43 caused marked elongation of microvilli (Fig. 11, D and E). Furthermore, introduction of Ez-T/D-VSVG, which mimicked activated ERM proteins, into serum-starved A431 cells but not L cells induced microvillar elongation. These findings suggest that in L cells the elongation of microvilli is regulated by the amount of ERMBMPs, whereas in serum-starved A431 cells it is regulated by the activation of ERM proteins as shown in Fig. 11. Based on these observations, we concluded that ERMBMPs such as CD43, CD44, and ICAM-2 can serve as organizing centers for microvilli in collaboration with activated ERM proteins. Of course, the role of ERMBMPs remains undetermined in the normal organization of microvilli in a variety of cells because our conclusion resulted from overexpression experiments. In support of our model, lymphocytes from patients with Wiskott-Aldrich syndrome, where CD43 is defective, were relatively devoid of microvilli (Kenney et al., 1986).

The question has naturally arisen as to the nature of the activated state of ERM proteins and how they are activated. Ezrin has been reported to be tyrosine-, serine-, and also threonine-phosphorylated in vivo. Ezrin is heavily tyrosine-phosphorylated in response to EGF in A431 and to hepatocyte growth factor in LLC-PK1 cells (Gould et al., 1986; Bretscher, 1989; Berryman et al., 1995; Crepaldi et al., 1997). However, tyrosine phosphorylation of ezrin did not alter the association of ezrin with the cytoskeleton (Gould et al., 1986; Egerton et al., 1992). Furthermore, even when two tyrosine residues in ezrin that are known to be major phosphorylation sites by EGF receptor (Krieg and Hunter, 1992) were mutated to phenylalanine, transfected LLC-PK1 cells became unresponsive to hepatocyte growth factor in terms of cell motility and tubulogenesis, but the localization of ezrin at microvilli was not affected (Crepaldi et al., 1997). These findings suggest that tyrosine phosphorylation of ezrin (and probably ERM proteins) is not required for ezrin activation as a membrane-cytoskeleton linker.

Several lines of evidence suggest that serine/threonine phosphorylation of ERM proteins is associated with their activation (Urushidani et al., 1989; Hanzel et al., 1991; Chen et al., 1995; Kondo et al., 1997; Oshiro et al., 1998; Shaw et al., 1998). Moesin was reported to be threonine-phosphorylated at its COOH terminus during thrombin activation in platelets (Nakamura et al., 1995). Phosphorylation at the COOH-terminal threonine residue was shown to affect the direct interaction between the NH₂- and COOH-terminal halves of radixin in vitro (Matsui et al., 1998), suggesting that this phosphorylation keeps ERM proteins in an opened (activated) form. The results of our previous investigations and of the present study indicated that CPERMs represent the activated ERM proteins shown in Fig. 11. Immunofluorescence microscopy with a mAb specific for CPERMs showed that they were highly concentrated at microvilli of cultured fibroblasts (Hayashi et al., 1999) and also at elongated microvilli of L and CV-1 cells overexpressing ERMBMPs (data not shown). When A431 cells were treated with EGF, levels of CPERMs were shown to be increased by immunoblotting, which were concentrated in elongating microvilli on the cell surface (Fig. 9), although it remains unclear how the activation of EGF receptor tyrosine kinase resulted in the phosphorylation of COOH-terminal threonine of ERM proteins. As expected, CPERMs were also highly concentrated at overelongated microvilli in EGF-treated A431 cells overexpressing ERMBMPs (data not shown). Furthermore, transfection experiments with site-directed mutants of ezrin revealed that Ez-T/D-VSVG (which mimicked CPERMs) was preferably recruited to the plasma membranes of serum-starved A431 cells to elongate microvilli. Similar results with mutated moesin were recently reported in COS7 cells (Oshiro et al., 1998). In contrast, Ez-T/A-VSVG, a nonphosphorylatable form at the COOH- terminal threonine, was codistributed with endogenous ERM proteins at the cytoplasm as well as plasma membranes (Fig. 10). Since ERM proteins form dimers or oligomers (Berryman et al., 1995; Bretscher et al., 1995), the localization of Ez-T/A-VSVG, i.e., inactivated form of ERM protein, on plasma membranes can be explained if these mutant molecules are incorporated into oligomers of endogenous ERM proteins.

Current knowledge regarding how ERM proteins are activated, i.e., how the COOH-terminal threonine residues of ERM proteins are phosphorylated in vivo, is still limited. Rho-associated kinase and/or protein kinase C-θ are good candidates for the kinase responsible for production of CPERMs in vivo (Matsui et al., 1998; Oshiro et al., 1998; Pietromonaco et al., 1998). Although protein kinase C-θ but not Rho-associated kinase was shown in vitro to phosphorylate full-length ezrin and moesin to directly open the closed form of ERM proteins (Simons et al., 1998), further analyses would be required to determine conclusively which serine/threonine kinase by itself functions as an activator for ERM proteins in vivo. Phosphoinositides such as phosphatidylinositol 4,5-bisphosphate were also thought to be key factors in opening of the closed ERM proteins (Niggli et al., 1995; Hirao et al., 1996). Further detailed analyses of how ERM proteins are activated in response to increases in amounts of ERMBMPs in fibroblasts and how this activation process is suppressed in epithelial cells will lead to a better understanding of the molecular mechanism of cell surface morphogenesis including the formation and destruction of microvilli in general.

We thank all the members of our laboratory (Department of Cell Biology, Faculty of Medicine, Kyoto University) for helpful discussions. We would like to express our appreciation to Mr. H. Maebashi (Laboratory of Electron Microscopy, National Institute for Physiological Sciences) for his assistance with scanning electron microscopy. The anti–E-cadherin pAb, mAb, and the plasmids pBATEM2 and pβ actCAT9 were gifts from Dr. M. Takeichi. We are also grateful to Dr. M. Miyasaka (Osaka University, Osaka, Japan) for his generous gift of anti–mouse CD44 mAb, IM7.8.1.

This study was supported in part by a Grant-in-Aid for Cancer Research and Grant-in-Aid for Scientific Research (A) from the Ministry of Education, Science and Culture of Japan.

CPERM

ERM protein phosphorylated at the COOH-terminal threonine residues

ERM

ezrin/radixin/ moesin

ERMBMP

ERM-binding membrane protein

pAb

polyclonal antibody

VSVG

vesicular stomatitis virus glycoprotein G