Bovine semitendinosus muscles were sampled immediately after death, after 24 hr postmortem with storage at 2°, 16°, or 37°C, and after 312 hr postmortem with storage at 2° and 16°C. A biopsy technique was used to prevent shortening during glutaraldehyde fixation. Postfixation in osmium tetroxide was followed by embedding in an Epon-Araldite mixture. Bovine muscle was supercontracted after 24 hr storage at 27deg; but was only slightly contracted after storage at 16° for 24 hr. Muscle held at 37° for 24 hr was slightly less supercontracted than the 2° muscle. Striking similarities existed between muscles stored at 16° and at 2°C for 312 hr. Both were slightly shortened with narrowed I bands and an area of increased density, probably due to overlap of thin filaments in the middle of the A band. Postmortem shortening was accompanied by banding-pattern changes similar to those predicted for contracting muscle by Huxley and Hanson's sliding filament model. Treatment of myofibrils with 0.05% trypsin resulted in a rapid loss of Z lines and, in supercontracted myofibrils, caused a return of the banding pattern of resting muscle.
Article|
August 01 1967
MORPHOLOGY OF RIGOR-SHORTENED BOVINE MUSCLE AND THE EFFECT OF TRYPSIN ON PRE- AND POSTRIGOR MYOFIBRILS
Marvin H. Stromer,
Marvin H. Stromer
From the Departments of Biochemistry and Biophysics and of Animal Science, the Iowa State University, Ames, Iowa 50010.
Search for other works by this author on:
D. E. Goll,
D. E. Goll
From the Departments of Biochemistry and Biophysics and of Animal Science, the Iowa State University, Ames, Iowa 50010.
Search for other works by this author on:
L. E. Roth
L. E. Roth
From the Departments of Biochemistry and Biophysics and of Animal Science, the Iowa State University, Ames, Iowa 50010.
Search for other works by this author on:
Marvin H. Stromer
From the Departments of Biochemistry and Biophysics and of Animal Science, the Iowa State University, Ames, Iowa 50010.
D. E. Goll
From the Departments of Biochemistry and Biophysics and of Animal Science, the Iowa State University, Ames, Iowa 50010.
L. E. Roth
From the Departments of Biochemistry and Biophysics and of Animal Science, the Iowa State University, Ames, Iowa 50010.
Dr. Stromer's present address is the Mellon Institute, Pittsburgh, Pennsylvania 15213. Dr. Goll's present address is the Cardiovascular Research Laboratory, the University of California Medical Center, Los Angeles, California 90024
Received:
November 07 1966
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1967 by The Rockefeller University Press
1967
J Cell Biol (1967) 34 (2): 431–445.
Article history
Received:
November 07 1966
Citation
Marvin H. Stromer, D. E. Goll, L. E. Roth; MORPHOLOGY OF RIGOR-SHORTENED BOVINE MUSCLE AND THE EFFECT OF TRYPSIN ON PRE- AND POSTRIGOR MYOFIBRILS . J Cell Biol 1 August 1967; 34 (2): 431–445. doi: https://doi.org/10.1083/jcb.34.2.431
Download citation file: