Swiss 3T3 cells exhibit contact-regulated cell growth and have a lower ability to transport 2-deoxyglucose than polyoma (Py)-transformed 3T3 cells. Py3T3 cells treated with dibutyryl cyclic adenosine 3'5' monophosphate (dBcAMP) and theophylline have reduced cell growth and transport 2-deoxyglucose at the same rate as normal 3T3 cells. Evidence that the cessation of cell growth and reduced transport abilities in Py3T3 cells does not represent a return to contact-regulated growth comes from the following observations. First, treating high density Py3T3 cells with dBcAMP allows more than two doublings of cell number, even though ability to transport 2-deoxyglucose is returned to levels equal to those of normal 3T3 cells. Second, dBcAMP prevents serum-stimulated increases in 2-deoxyglucose transport in Py3T3 but not in 3T3 cells.
DIBUTYRYL CYCLIC ADENOSINE 3' 5' MONOPHOSPHATE, SUGAR TRANSPORT, AND REGULATORY CONTROL OF CELL DIVISION IN NORMAL AND TRANSFORMED CELLS
William J. Grimes, Judith L. Schroeder; DIBUTYRYL CYCLIC ADENOSINE 3' 5' MONOPHOSPHATE, SUGAR TRANSPORT, AND REGULATORY CONTROL OF CELL DIVISION IN NORMAL AND TRANSFORMED CELLS . J Cell Biol 1 February 1973; 56 (2): 487–491. doi: https://doi.org/10.1083/jcb.56.2.487
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