The requirement for Zn++ in DNA replication by phytohemagglutinin-stimulated human lymphocytes was studied. When 6 µM o-phenanthroline, a chelator with a high affinity for Zn++, is added to cultures of stimulated lymphocytes a nearly complete inhibition of thymidine incorporation results within a few hours. In contrast, the incorporation of uridine is only slightly reduced and the incorporation of leucine is unaffected. m-Phenanthroline, a nonchelating analogue, does not alter the rate of thymidine incorporation even when present in 10-fold greater amounts than o-phenanthroline. The inhibition of thymidine incorporation by o-phenanthroline could be entirely reversed by the addition of Zn++ to the cultures, or could be prevented by the prior addition of either Zn++ or Ni++. All other divalent cations tested were incapable of reversing the o-phenanthroline inhibition of thymidine incorporation.
Article|
September 01 1973
ZINC REQUIREMENT FOR DNA REPLICATION IN STIMULATED HUMAN LYMPHOCYTES
Richard O. Williams,
Richard O. Williams
From The Institute for Cancer Research, Fox Chase Center for Cancer and Medical Sciences, Philadelphia, Pennsylvania 19111
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Lawrence A. Loeb
Lawrence A. Loeb
From The Institute for Cancer Research, Fox Chase Center for Cancer and Medical Sciences, Philadelphia, Pennsylvania 19111
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Richard O. Williams
From The Institute for Cancer Research, Fox Chase Center for Cancer and Medical Sciences, Philadelphia, Pennsylvania 19111
Lawrence A. Loeb
From The Institute for Cancer Research, Fox Chase Center for Cancer and Medical Sciences, Philadelphia, Pennsylvania 19111
Received:
March 08 1973
Revision Received:
May 21 1973
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1973 by The Rockefeller University Press
1973
J Cell Biol (1973) 58 (3): 594–601.
Article history
Received:
March 08 1973
Revision Received:
May 21 1973
Citation
Richard O. Williams, Lawrence A. Loeb; ZINC REQUIREMENT FOR DNA REPLICATION IN STIMULATED HUMAN LYMPHOCYTES . J Cell Biol 1 September 1973; 58 (3): 594–601. doi: https://doi.org/10.1083/jcb.58.3.594
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