Acetylcholine receptors were inactivated in vivo at the mouse neuromuscular junction using alpha-bungarotoxin (alpha-BTX). It was found that neurally produced muscle contraction recovered within 4-8 days (halftime similar to 3 days). Actinomycin D interfered with this recovery, but did not affect normal nerve-stimulated muscle contraction. If the response was initially eliminated by [125-I]alpha-BTX and the end plates examined by EM autoradiography, no evidence of mass internalization of bound radioactivity during recovery was seen. The fine structure of the end plates and muscle was unaltered during the post-alpha-BTX recovery period.
Article| July 01 1975
In vivo recovery of muscle contraction after alpha-bungarotoxin binding.
H C Fertuck
M M Salpeter
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1975) 66 (1): 209–213.
H C Fertuck, W Woodward, M M Salpeter; In vivo recovery of muscle contraction after alpha-bungarotoxin binding.. J Cell Biol 1 July 1975; 66 (1): 209–213. doi: https://doi.org/10.1083/jcb.66.1.209
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