Washed mature spermatozoa from bulls incorporate ribonucleoside triphosphates into RNA using an endogenous template. Maximum incorporation was observed at 31 degrees C in the presence of MgCl2, all four ribonucleoside triphosphates, beta-mercaptoethanol, and glycine sodium hydroxide buffer at pH 9.0. The amount of synthesis was linearly dependent upon the concentration of spermatozoa and continued for at least 4 h. Digestion studies revealed the RNA to be present in a protected (intracellular?) location in the spermatozoa. The RNA synthesis was inhibited by ethidium bromide, rifampicin, acriflavine, actinomycin D, and caffeine, but not by alpha-amanitine or rifamycin SV. Fractionation of the spermatozoa by sonication and separation of the heads and tails by centrifugation through a discontinuous gradient revealed that more than half of the total RNA polymerase activity was associated with the tail fraction.
Article| September 01 1977
RNA polymerase activity in bovine spermatozoa.
C D Fuster
F A Stern
N B Hecht
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1977) 74 (3): 698–706.
C D Fuster, D Farrell, F A Stern, N B Hecht; RNA polymerase activity in bovine spermatozoa.. J Cell Biol 1 September 1977; 74 (3): 698–706. doi: https://doi.org/10.1083/jcb.74.3.698
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