We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double-staining experiments the localization of the 110-kdalton protein by the JLJ5a monoclonal antibody was coincident with the reaction products of thiamine pyrophosphatase (one of the enzyme markers of the Golgi apparatus; Novikoff and Goldfischer, 1961, Proc. Natl. Acad. Sci. U.S.A. 47:802-810) in the same cells. (b) The staining pattern of the JLJ5a monoclonal antibody became fragmented and dispersed into vacuoles after pretreatment of the cells with Colcemid or monensin.
Article| January 01 1982
A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.
J J Lin
S A Queally
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1982) 92 (1): 108–112.
J J Lin, S A Queally; A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.. J Cell Biol 1 January 1982; 92 (1): 108–112. doi: https://doi.org/10.1083/jcb.92.1.108
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