An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with colloidal gold conjugated second antibody. The endogenous mammalian lectin was found in smooth muscle cells and squamous alveolar epithelial (type I) cells and was concentrated extracellularly in elastic fibers of pulmonary parenchyma and blood vessels. The extracellular localization of this lectin suggests that it, like others, functions by interaction with extracellular glycoconjugates.
Article| April 01 1984
Endogenous mammalian lectin localized extracellularly in lung elastic fibers.
P L Haywood-Reid,
R F Cerra
P L Haywood-Reid
S H Barondes
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1984) 98 (4): 1580–1589.
R F Cerra, P L Haywood-Reid, S H Barondes; Endogenous mammalian lectin localized extracellularly in lung elastic fibers.. J Cell Biol 1 April 1984; 98 (4): 1580–1589. doi: https://doi.org/10.1083/jcb.98.4.1580
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