On the cover
The mitotic spindle (orange) segregates DNA (blue) in the dividing cells of a sand dollar embryo. von Dassow et al. demonstrate that spindle microtubules don't need to touch the cell cortex for the cleavage furrow to assemble in the right place.
See page 831.
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In This Issue
Sculpting the dynein regulatory complex
Cryoelectron tomography materializes the (missing) nexin link.
People & Ideas
Tarun Kapoor: In the right position to study chromosomes
Kapoor uses small molecule inhibitors and high-resolution microscopy to explore metaphase chromosome alignment.
Overall Cdk activity modulates the DNA damage response in mammalian cells
It's the degree of activation, not a specific cyclin-dependent kinase, that is important for genome maintenance in postreplicative cells.
Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase
A new technique illuminates differential H3S10 phosphorylation dynamics in normal and cancer cells; spatial and temporal regulation of this process by aurora B kinase is required for accurate chromosome segregation.
The Cul3–KLHL21 E3 ubiquitin ligase targets Aurora B to midzone microtubules in anaphase and is required for cytokinesis
Selective ubiquitination of Aurora B by different Cul3 adaptors targets it at the correct time to the correct place during mitosis.
Disease-specific gene repositioning in breast cancer
The nuclear repositioning of specific genes may be a novel diagnostic strategy to distinguish malignant from normal tissue.
ISWI is a RanGTP-dependent MAP required for chromosome segregation
Chromatin-remodeling factor ISWI is a microtubule-associated protein that contributes to chromosome segregation by stabilizing microtubules during anaphase.
Action at a distance during cytokinesis
Astral microtubule contact with the cortex is not required to position the furrow for cytokinesis.
Dual roles for the Drosophila PI 4-kinase Four wheel drive in localizing Rab11 during cytokinesis
Fwd shuttles Rab11 to the cleavage furrow by both kinase-dependent and -independent mechanisms.
Muscle inactivation of mTOR causes metabolic and dystrophin defects leading to severe myopathy
mTor, acting mainly via mTORC1, controls dystrophin transcription in a raptor- and rictor-independent mechanism.
Valosin-containing protein (VCP) is required for autophagy and is disrupted in VCP disease
Accumulation of autophagosomes because of impaired autophagy during valosin-containing protein (VCP)–linked dementia is explained by the absence or reduced activity of VCP.
Lipid-regulated sterol transfer between closely apposed membranes by oxysterol-binding protein homologues
The ORP lipid-binding domain can contact two membranes simultaneously to facilitate sterol extraction or delivery at one membrane in response to the lipid composition of the other.
Basal body stability and ciliogenesis requires the conserved component Poc1
Poc1 shores up basal bodies to support cilia formation in Tetrahymena thermophila, zebrafish, and humans; Poc1 depletion causes phenotypes commonly seen in ciliopathies.
The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella
Elegant cryoelectron tomography reveals that the nexin link between microtubule doublets in 9 + 2 axonemal structures, critical for their ability to bend, is the dynein regulatory complex.