On the cover
In the lung of an E14.5 mouse embryo, β-galactosidase staining (red) labels fibroblasts expressing the secreted Hedgehog antagonist HHIP1 (green), which not only associates with these cells but also with the basement membrane underlying the lung epithelium. DNA is labeled blue.
Image ©2015 Holtz et al.
See page 739.
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
In This Issue
How chromosomes shrink to fit
Chromosome length in early embryo reflects the dimensions of the cell and nucleus.
People & Ideas
Junjie Hu: Shape-shifting in the endoplasmic reticulum
Hu investigates proteins that bend and blend ER membranes.
Mitotic chromosome length scales in response to both cell and nuclear size
Quantitative analysis relating cell, nuclear, and chromosome size in C. elegans embryos predicts two levels of chromosome length regulation in response to both cell and nuclear size.
Alternative splicing converts STIM2 from an activator to an inhibitor of store-operated calcium channels
STIM2β is a novel STIM2 splice isoform that inhibits Orai channels.
KCC2 regulates actin dynamics in dendritic spines via interaction with β-PIX
In neurons, KCC2 controls glutamatergic synaptogenesis and regulates actin polymerization in dendritic spines by binding β-PIXb and attenuating its GEF activity toward small GTPase Rac1.
Diffusion and retention are major determinants of protein targeting to the inner nuclear membrane
Data from a new in vitro assay measuring inner nuclear membrane (INM) targeting kinetics as well as from mathematical modeling support a diffusion-retention–based mechanism of INM targeting.
Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells
A new reporter allowing real-time imaging of membrane protein transport from the ER to the inner nuclear membrane (INM) reveals that targeting of INM proteins depends on the number and permeability of nuclear pores and the availability of nuclear binding sites.
Erbin is a novel substrate of the Sag-βTrCP E3 ligase that regulates KrasG12D-induced skin tumorigenesis
In contrast to previous results in the lung, skin-specific deletion of the Sag-βTrCP E3 ubiquitin ligase significantly accelerates mutant KrasG12D-induced skin papillomagenesis due to accumulation of Erbin and Nrf2, two novel Sag substrates, which blocks ROS generation and promotes proliferation.
Secreted HHIP1 interacts with heparan sulfate and regulates Hedgehog ligand localization and function
HHIP1 acts as a secreted Hedgehog antagonist whose association with heparan sulfate–containing basement membranes regulates Hedgehog ligand distribution.
Spatiotemporal dynamics of traction forces show three contraction centers in migratory neurons
Traction force microscopy provides a comprehensive description of the spatiotemporal dynamics of contractile activities and their regulation by guidance molecules in migrating neurons, as well as the underlying molecular mechanisms.