Rabbit antisera against normal guinea pig lymph node, when injected into guinea pigs, produced transient depression of the level of blood lymphocytes. It had no effect on other circulating cellular elements. Repeated injection over several days produced lymphopenia, which became progressively less marked with continued treatment, and clear-cut depletion of small lymphocytes in lymph nodes, whether draining an inoculation site or remote. In guinea pigs treated with lymphocyte antiserum, there was marked suppression of the tuberculin and contact allergic reactions and the "delayed" skin reaction to purified diphtheria toxoid, and a relative suppression of allergic encephalomyelitis and the rejection of first set skin homografts. There was a slight effect on second set graft rejection and no effect on PCA or the reversed passive Arthus reaction. Non-specific reactions to intradermal turpentine or to concentrated dinitrochlorobenzene placed on the skin were moderately reduced. The suppression of these reactions (except allergic encephalomyelitis) was closely correlated with the degree of lymphopenia. Lymphocyte antiserum absorbed with normal blood white cells lost both its lymphopenic effect and its ability to suppress the tuberculin reaction. It is tentatively concluded that a circulating mononuclear cell, probably the small lymphocyte, is the primary reactant in the various types of delayed hypersensitive reactions.
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December 01 1961
THE USE OF SPECIFIC "LYMPHOCYTE" ANTISERA TO INHIBIT HYPERSENSITIVE REACTIONS OF THE "DELAYED" TYPE
Byron H. Waksman,
Byron H. Waksman
From the Department of Bacteriology and Immunology, Harvard Medical School, and the Neurology Service, Massachusetts General Hospital, Boston
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Simone Arbouys,
Simone Arbouys
From the Department of Bacteriology and Immunology, Harvard Medical School, and the Neurology Service, Massachusetts General Hospital, Boston
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Barry G. Arnason
Barry G. Arnason
From the Department of Bacteriology and Immunology, Harvard Medical School, and the Neurology Service, Massachusetts General Hospital, Boston
Search for other works by this author on:
Byron H. Waksman
From the Department of Bacteriology and Immunology, Harvard Medical School, and the Neurology Service, Massachusetts General Hospital, Boston
Simone Arbouys
From the Department of Bacteriology and Immunology, Harvard Medical School, and the Neurology Service, Massachusetts General Hospital, Boston
Barry G. Arnason
From the Department of Bacteriology and Immunology, Harvard Medical School, and the Neurology Service, Massachusetts General Hospital, Boston
Received:
June 13 1961
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1961, by The Rockefeller Institute
1961
J Exp Med (1961) 114 (6): 997–1022.
Article history
Received:
June 13 1961
Citation
Byron H. Waksman, Simone Arbouys, Barry G. Arnason; THE USE OF SPECIFIC "LYMPHOCYTE" ANTISERA TO INHIBIT HYPERSENSITIVE REACTIONS OF THE "DELAYED" TYPE . J Exp Med 1 December 1961; 114 (6): 997–1022. doi: https://doi.org/10.1084/jem.114.6.997
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