Antigenic modulation (the loss of TL antigens from TL+ cells exposed to TL antibody in the absence of lytic complement) has been demonstrated in vitro. An ascites leukemia, phenotype TL.1,2,3, which modulates rapidly and completely when incubated with TL antiserum in vitro, was selected for further study of the phenomenon. Over a wide range of TL antibody concentrations modulation at 37°C was detectable within 10 min and was complete within approximately 1 hr. The cells were initially sensitized to C' by their contact with antibody, thereafter losing this sensitivity to C' lysis together with their sensitivity to TL antibody and C' in the cytotoxic test. The capacity of the cells to undergo modulation was abolished by actinomycin D and by iodoacetamide, and by reducing the temperature of incubation to 0°C. Thus modulation apparently is an active cellular process. Antigens TL. 1,2, and 3 are all modulated by anti-TL.1,3 serum and by anti-TL.3 serum. This modulation affects all three TL components together, even when antibody to one or two of them is lacking. aAnti-TL.2 serum does not induce modulation and in fact impairs modulation by the other TL antibodies. The influence of the TL phenotype of cells upon the demonstrable content of H-2 (D region) isoantigen, first shown in cells modulated in vivo, has been observed with cells modulated in vitro. Cells undergoing modulation show a progressive increase in H-2 (D region) antigen over a period of 4 hr, with no change in H-2 antigens of the K region. Restoration of the TL+ phenotype of modulated cells after removal of antibody is less rapid than TL+ → TL- modulation and may require several cell divisions.
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March 01 1968
ANTIGENIC MODULATION : LOSS OF TL ANTIGEN FROM CELLS EXPOSED TO TL ANTIBODY. STUDY OF THE PHENOMENON IN VITRO
Lloyd J. Old,
Lloyd J. Old
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
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Elisabeth Stockert,
Elisabeth Stockert
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
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Edward A. Boyse,
Edward A. Boyse
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
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Jae Ho Kim
Jae Ho Kim
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
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Lloyd J. Old
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
Elisabeth Stockert
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
Edward A. Boyse
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
Jae Ho Kim
From the Divisions of Immunology and Biophysics, Sloan-Kettering Institute for Cancer Research, and Cornell University Graduate School of Medical Sciences, Cornell University Medical College, New York 10021
Received:
November 05 1967
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1968 by The Rockefeller University Press
1968
J Exp Med (1968) 127 (3): 523–539.
Article history
Received:
November 05 1967
Citation
Lloyd J. Old, Elisabeth Stockert, Edward A. Boyse, Jae Ho Kim; ANTIGENIC MODULATION : LOSS OF TL ANTIGEN FROM CELLS EXPOSED TO TL ANTIBODY. STUDY OF THE PHENOMENON IN VITRO . J Exp Med 1 March 1968; 127 (3): 523–539. doi: https://doi.org/10.1084/jem.127.3.523
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