Quantitation of surface and total cell Ig obtained after lysis by detergent, urea-acid treatment, and freeze-thawing were determined on spleen cells, thymus cells, and spleen cells specifically depleted of B cells. A two- to four-fold increase in measurable Ig was found after cell lysis. All cell populations showed a similar increase in measurable Ig indicating that no discordantly large amounts of buried Ig determinants were associated with the surface of T cells. The lack of appreciable amounts of T cell Ig was confirmed by immunoprecipitation of radioiodinated cells. A theta-positive lymphoma was described which, when grown in culture, lacked detectable surface Ig but contained a receptor site for IgG. This resulted in appreciable amounts of surface IgG being associated with the tumor line when isolated from ascitic fluid of tumor-bearing mice or after preincubation of cultured cells with either heat-aggregated IgG or normal mouse serum.
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November 01 1972
THYMUS-DERIVED (T) CELL IMMUNOGLOBULINS : PRESENCE OF A RECEPTOR SITE FOR IGG AND ABSENCE OF LARGE AMOUNTS OF "BURIED" IG DETERMINANTS ON T CELLS
Howard M. Grey,
Howard M. Grey
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206, and the Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
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Ralph T. Kubo,
Ralph T. Kubo
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206, and the Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
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Jean-Charles Cerottini
Jean-Charles Cerottini
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206, and the Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
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Howard M. Grey
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206, and the Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
Ralph T. Kubo
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206, and the Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
Jean-Charles Cerottini
From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206, and the Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
Received:
August 25 1972
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1972 by The Rockefeller University Press
1972
J Exp Med (1972) 136 (5): 1323–1328.
Article history
Received:
August 25 1972
Citation
Howard M. Grey, Ralph T. Kubo, Jean-Charles Cerottini; THYMUS-DERIVED (T) CELL IMMUNOGLOBULINS : PRESENCE OF A RECEPTOR SITE FOR IGG AND ABSENCE OF LARGE AMOUNTS OF "BURIED" IG DETERMINANTS ON T CELLS . J Exp Med 1 November 1972; 136 (5): 1323–1328. doi: https://doi.org/10.1084/jem.136.5.1323
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