Lipopolysaccharides with different structure, isolated from different mutant strains of Escherichia coli and Salmonella bacteria, and chemical degradation products of these lipopolysaccharides have been employed to investigate which part of the lipopolysaccharide molecule exerts mitogenic effects on bone marrow-derived mouse lymphocytes. Within the structure of lipopolysaccharide consisting of lipid A, a core polysaccharide, and the O-polysaccharide antigen, lipid A was found to be the mitogenic part. The mitogenic effect of lipid A, consisting of phosphorylated glucosamine disaccharide units with ester- and amide-linked fatty acids, was lost after alkali treatment, which removes ester-linked fatty acids. Insertion of the lipid A portion of lipopolysaccharides into the lipid bilayer of the plasma membranes of bone marrow-derived lymphocytes is discussed as the initial mitogenic action.
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April 01 1973
THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE
Jan Andersson,
Jan Andersson
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
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Fritz Melchers,
Fritz Melchers
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
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Chris Galanos,
Chris Galanos
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
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Otto Lüderitz
Otto Lüderitz
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
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Jan Andersson
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
Fritz Melchers
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
Chris Galanos
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
Otto Lüderitz
From the Basel Institute for Immunology, Basel, Switzerland, and the Max-Planck-Institut für Immunobiologie, Freiburg, Germany
Received:
December 10 1972
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 137 (4): 943–953.
Article history
Received:
December 10 1972
Citation
Jan Andersson, Fritz Melchers, Chris Galanos, Otto Lüderitz; THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE . J Exp Med 1 April 1973; 137 (4): 943–953. doi: https://doi.org/10.1084/jem.137.4.943
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