A number of inbred and congenic resistant strains of mice were immunized with staphylococcal nuclease (Nase). Antibody responses were measured in the sera of the animals by a sensitive method involving inhibition of enzymatic hydrolysis of DNA, High responder strains included A/J, DBA/2, BALB/c, AKR/J, C57BR, and SJL/J. DBA/1 and C57BL/6 mice were low responders. The strain distribution of anti-Nase response potential was compatible with the relevant immune response gene(s) being linked to the murine major histocompatibility complex. Linkage of this response to H-2 was demonstrated by the findings that: (a) the congenic C3H/HeJ and C3H.SW mice were respectively high and low responders; (b) the congenic lines B10.A and B10.D2 were high responders, whereas the C57BL/10 strain was a poor responder; and (c) anti-Nase response potential of F2 progeny from DBA/1 x SJL/J matings correlated with their H-2 type. Three B10.A recombinant lines were used to map this Ir gene within H-2. B10.A(4R) was a high responder to Nase, whereas B10.A(2R) and B10.A(5R) were both low responders. We wish to propose the name Ir-Nase for the gene(s) controlling antibody responsiveness to this immunogen. Our data indicate that Ir-Nase is located within the same chromosomal segment of the H-2 complex as is Ir-IgG.
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May 01 1974
GENETIC CONTROL OF THE IMMUNE RESPONSE TO STAPHYLOCOCCAL NUCLEASE : I. IR-NASE: CONTROL OF THE ANTIBODY RESPONSE TO NUCLEASE BY THE IR REGION OF THE MOUSE H-2 COMPLEX
Eugene C. Lozner,
Eugene C. Lozner
From the Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014
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David H. Sachs,
David H. Sachs
From the Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014
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Gene M. Shearer
Gene M. Shearer
From the Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014
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Eugene C. Lozner
From the Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014
David H. Sachs
From the Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014
Gene M. Shearer
From the Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014
Received:
January 28 1974
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1974 by The Rockefeller University Press
1974
J Exp Med (1974) 139 (5): 1204–1214.
Article history
Received:
January 28 1974
Citation
Eugene C. Lozner, David H. Sachs, Gene M. Shearer; GENETIC CONTROL OF THE IMMUNE RESPONSE TO STAPHYLOCOCCAL NUCLEASE : I. IR-NASE: CONTROL OF THE ANTIBODY RESPONSE TO NUCLEASE BY THE IR REGION OF THE MOUSE H-2 COMPLEX . J Exp Med 1 May 1974; 139 (5): 1204–1214. doi: https://doi.org/10.1084/jem.139.5.1204
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