Prior work has shown that purified, resident, and inflammatory peritoneal macrophages are weak stimulators of the allogeneic MLR. We have identified conditions whereby thioglycollate-elicited macrophages become stimulatory, but primarily for the CD8+ T cell subset. The conditions were to treat the macrophages with neuraminidase and to supplement the MLR with rIL-2. These treatments together led to proliferative and cytotoxic responses by isolated CD8+ but not CD4+ T cells. Likewise when MHC-congenic strains were evaluated, an MLR was observed across isolated class I but not class II MHC barriers. Pretreatment of the macrophages with IFN-gamma further enhanced expression of class I MHC products and stimulatory activity, but did not seem essential. While these treatments did not render macrophages stimulatory for an MLR in purified CD4+ cells, blastogenesis of CD4+ cells was observed when the MLR involved bulk T cells. Small allogeneic B lymphocytes behaved similarly to macrophages, in the pretreatment with neuraminidase and supplementation with rIL-2 rendered B cells stimulatory for allogeneic, enriched, CD8+, but not CD4+, T cells. Spleen adherent cells, which are mixtures of macrophages and dendritic cells, stimulated both CD4+ and CD8+ T cells, and neither neuraminidase nor exogenous IL-2 was required. We think that these data suggest that most macrophages and small B cells lack three important functions of dendritic cells: a T cell-binding function that can be remedied by neuraminidase treatment, a T cell growth factor-inducing function that can be bypassed with exogenous IL-2, and an IL-2 responsiveness function that is required by CD4+ lymphocytes.
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October 01 1988
Neuraminidase-treated macrophages stimulate allogenic CD8+ T cells in the presence of exogenous interleukin 2.
Y Hirayama,
Y Hirayama
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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K Inaba,
K Inaba
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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M Inaba,
M Inaba
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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T Kato,
T Kato
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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M Kitaura,
M Kitaura
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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T Hosokawa,
T Hosokawa
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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S Ikehara,
S Ikehara
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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S Muramatsu
S Muramatsu
Department of Zoology, Faculty of Science, Kyoto University, Japan.
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Y Hirayama
Department of Zoology, Faculty of Science, Kyoto University, Japan.
K Inaba
Department of Zoology, Faculty of Science, Kyoto University, Japan.
M Inaba
Department of Zoology, Faculty of Science, Kyoto University, Japan.
T Kato
Department of Zoology, Faculty of Science, Kyoto University, Japan.
M Kitaura
Department of Zoology, Faculty of Science, Kyoto University, Japan.
T Hosokawa
Department of Zoology, Faculty of Science, Kyoto University, Japan.
S Ikehara
Department of Zoology, Faculty of Science, Kyoto University, Japan.
S Muramatsu
Department of Zoology, Faculty of Science, Kyoto University, Japan.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1988) 168 (4): 1443–1456.
Citation
Y Hirayama, K Inaba, M Inaba, T Kato, M Kitaura, T Hosokawa, S Ikehara, S Muramatsu; Neuraminidase-treated macrophages stimulate allogenic CD8+ T cells in the presence of exogenous interleukin 2.. J Exp Med 1 October 1988; 168 (4): 1443–1456. doi: https://doi.org/10.1084/jem.168.4.1443
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