While all known microbial superantigens are mitogenic for human peripheral blood lymphocytes (PBL), the functional response induced by Mycoplasma arthritidis-derived superantigen (MAM) is unique in that MAM stimulation of PBL consistently results in T cell-dependent B cell activation characterized by polyclonal IgM and IgG production. These immunostimulatory effects of MAM on the humoral arm of the human immune system warranted a more precise characterization of MAM-reactive human T cells. Using an uncloned MAM reactive human T cell line as immunogen, we have generated a monoclonal antibody (mAb) (termed C1) specific for the T cell receptor V beta gene expressed by the major fraction of MAM-reactive human T cells, V beta 17. In addition, a V beta 17- MAM-reactive T cell population exists, assessed by MAM, induced T cell proliferation and cytotoxic T cell activity. mAb C1 will be useful in characterizing the functional properties of V beta 17+ T cells and their potential role in autoimmune disease.
Skip Nav Destination
Article navigation
Article|
October 01 1991
Characterization of human T cells reactive with the Mycoplasma arthritidis-derived superantigen (MAM): generation of a monoclonal antibody against V beta 17, the T cell receptor gene product expressed by a large fraction of MAM-reactive human T cells.
S M Friedman,
S M Friedman
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
M K Crow,
M K Crow
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
J R Tumang,
J R Tumang
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
M Tumang,
M Tumang
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
Y Q Xu,
Y Q Xu
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
A S Hodtsev,
A S Hodtsev
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
B C Cole,
B C Cole
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
D N Posnett
D N Posnett
Department of Medicine, Hospital for Special Surgery, New York 10021.
Search for other works by this author on:
S M Friedman
Department of Medicine, Hospital for Special Surgery, New York 10021.
M K Crow
Department of Medicine, Hospital for Special Surgery, New York 10021.
J R Tumang
Department of Medicine, Hospital for Special Surgery, New York 10021.
M Tumang
Department of Medicine, Hospital for Special Surgery, New York 10021.
Y Q Xu
Department of Medicine, Hospital for Special Surgery, New York 10021.
A S Hodtsev
Department of Medicine, Hospital for Special Surgery, New York 10021.
B C Cole
Department of Medicine, Hospital for Special Surgery, New York 10021.
D N Posnett
Department of Medicine, Hospital for Special Surgery, New York 10021.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 174 (4): 891–900.
Citation
S M Friedman, M K Crow, J R Tumang, M Tumang, Y Q Xu, A S Hodtsev, B C Cole, D N Posnett; Characterization of human T cells reactive with the Mycoplasma arthritidis-derived superantigen (MAM): generation of a monoclonal antibody against V beta 17, the T cell receptor gene product expressed by a large fraction of MAM-reactive human T cells.. J Exp Med 1 October 1991; 174 (4): 891–900. doi: https://doi.org/10.1084/jem.174.4.891
Download citation file:
Suggested Content
Email alerts
Advertisement