Mice harboring a deletion of the gene encoding the transporter associated with antigen presentation-1 (TAP1) are impaired in providing major histocompatibility complex (MHC) class I molecules with peptides of cytosolic origin and lack stable MHC class I cell surface expression. They consequently have a strongly reduced number of CD8+ T cells. To examine whether selection of CD8+ T cells is dependent on TAP-dependent peptides, we partially restored MHC class I cell surface expression in TAP1-deficient mice by introduction of human beta 2-microglobulin. We show that selection of functional CD8+ T cells can be augmented in vivo in the absence of TAP1-dependent peptides.
Increase in positive selection of CD8+ T cells in TAP1-mutant mice by human beta 2-microglobulin transgene.
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H Martien van Santen, A Woolsey, P G Rickardt, L Van Kaer, E J Baas, A Berns, S Tonegawa, H L Ploegh; Increase in positive selection of CD8+ T cells in TAP1-mutant mice by human beta 2-microglobulin transgene.. J Exp Med 1 February 1995; 181 (2): 787–792. doi: https://doi.org/10.1084/jem.181.2.787
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