We have examined the extent of allelic exclusion at the T cell receptor (TCR) beta locus using monoclonal antibodies specific for V beta products. A small proportion (approximately 1%) of human peripheral blood T cells express two V beta as determined by flow cytometric analysis, isolation of representative clones, and sequencing of the corresponding V beta chains. Dual beta T cells are present in both the CD45R0+ and CD45R0- subset. These results indicate that dual beta expression is compatible with both central and peripheral selection. They also suggest that the substantial degree of TCR beta allelic exclusion is dependent only on asynchronous rearrangements at the beta locus, whereas the role of the pre-TCR is limited to signaling the presence of at least one functional beta protein.
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April 01 1995
Normal T lymphocytes can express two different T cell receptor beta chains: implications for the mechanism of allelic exclusion.
E Padovan,
E Padovan
Basel Institute for Immunology, Switzerland.
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C Giachino,
C Giachino
Basel Institute for Immunology, Switzerland.
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M Cella,
M Cella
Basel Institute for Immunology, Switzerland.
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S Valitutti,
S Valitutti
Basel Institute for Immunology, Switzerland.
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O Acuto,
O Acuto
Basel Institute for Immunology, Switzerland.
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A Lanzavecchia
A Lanzavecchia
Basel Institute for Immunology, Switzerland.
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E Padovan
Basel Institute for Immunology, Switzerland.
C Giachino
Basel Institute for Immunology, Switzerland.
M Cella
Basel Institute for Immunology, Switzerland.
S Valitutti
Basel Institute for Immunology, Switzerland.
O Acuto
Basel Institute for Immunology, Switzerland.
A Lanzavecchia
Basel Institute for Immunology, Switzerland.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1995) 181 (4): 1587–1591.
Citation
E Padovan, C Giachino, M Cella, S Valitutti, O Acuto, A Lanzavecchia; Normal T lymphocytes can express two different T cell receptor beta chains: implications for the mechanism of allelic exclusion.. J Exp Med 1 April 1995; 181 (4): 1587–1591. doi: https://doi.org/10.1084/jem.181.4.1587
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