The morphologic changes occurring in Ehrlich ascites tumor cells infected with chick embryo-adapted Newcastle disease virus were studied with phase microscope, conventional light microscope, and electron microscope. Intracytoplasmic inclusions appeared 2 to 4 hours following infection and progressively increased in size and numbers until cytolysis occurred. No significant alterations in mitochondria or other cell organelles were detected during the initial period of inclusion development. The inclusions were composed of a multilaminated shell, probably derived from the agranular reticulum of the EAT cell, surrounding an inner core packed with dense particles, measuring 3 to 14 mµ in diameter. These particles were tentatively identified as the newly synthesized "incomplete virus" which had been previously demonstrated by fluorescent antibody techniques. The possible role of the inclusions is discussed.
Article|
November 01 1957
AN ELECTRON MICROSCOPIC STUDY OF INCOMPLETE VIRUS FORMATION : INFECTION OF EHRLICH ASCITES TUMOR CELLS WITH "CHICK EMBRYO-ADAPTED" NEWCASTLE DISEASE VIRUS (NDV)
W. Robert Adams,
W. Robert Adams
From the Department of Pathology, Yale University School of Medicine, New Haven
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Alfred M. Prince
Alfred M. Prince
From the Department of Pathology, Yale University School of Medicine, New Haven
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W. Robert Adams
From the Department of Pathology, Yale University School of Medicine, New Haven
Alfred M. Prince
From the Department of Pathology, Yale University School of Medicine, New Haven
Received:
May 31 1957
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1957, by The Rockefeller Institute for Medical Research New York
1957
J Exp Med (1957) 106 (5): 617–626.
Article history
Received:
May 31 1957
Citation
W. Robert Adams, Alfred M. Prince; AN ELECTRON MICROSCOPIC STUDY OF INCOMPLETE VIRUS FORMATION : INFECTION OF EHRLICH ASCITES TUMOR CELLS WITH "CHICK EMBRYO-ADAPTED" NEWCASTLE DISEASE VIRUS (NDV) . J Exp Med 1 November 1957; 106 (5): 617–626. doi: https://doi.org/10.1084/jem.106.5.617
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