Primary or established strain cultures of a variety of primate cells that were susceptible to cytopathic infection strongly adsorbed poliovirus. Insusceptible non-primate cells in primary or established-strain culture did not so adsorb virus (or propagate it), with exception of the ERK-1 embryo rabbit kidney strain. All tested cells, regardless of type or susceptibility, adsorbed about 1 per cent of input virus, which became cell-associated (CAV) without loss of infectivity. In combination with susceptible or insusceptible cells, CAV was only about 90 per cent neutralized by homotypic antiserum. CAV eluted continuously from non-susceptible cells with continued incubation; eluted virus gave rise to infection and new CAV to the same degree in susceptible cells as did original virus.
Article|
May 01 1959
THE MAMMALIAN CELL-VIRUS RELATIONSHIP : I. ATTACHMENT OF POLIOVIRUS TO CULTIVATED CELLS OF PRIMATE AND NON-PRIMATE ORIGIN
Leroy C. McLaren,
Leroy C. McLaren
From the Department of Bacteriology and Immunology, University of Minnesota, Minneapolis
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John J. Holland,
John J. Holland
From the Department of Bacteriology and Immunology, University of Minnesota, Minneapolis
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Jerome T. Syverton
Jerome T. Syverton
From the Department of Bacteriology and Immunology, University of Minnesota, Minneapolis
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Leroy C. McLaren
From the Department of Bacteriology and Immunology, University of Minnesota, Minneapolis
John J. Holland
From the Department of Bacteriology and Immunology, University of Minnesota, Minneapolis
Jerome T. Syverton
From the Department of Bacteriology and Immunology, University of Minnesota, Minneapolis
Received:
January 18 1959
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1959, by The Rockefeller Institute
1959
J Exp Med (1959) 109 (5): 475–485.
Article history
Received:
January 18 1959
Citation
Leroy C. McLaren, John J. Holland, Jerome T. Syverton; THE MAMMALIAN CELL-VIRUS RELATIONSHIP : I. ATTACHMENT OF POLIOVIRUS TO CULTIVATED CELLS OF PRIMATE AND NON-PRIMATE ORIGIN . J Exp Med 1 May 1959; 109 (5): 475–485. doi: https://doi.org/10.1084/jem.109.5.475
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