A model system for the investigation of immune cellular injury in primary isolates of a fixed-tissue cell of human origin has been described, using metabolism-inhibition and uptake of trypan blue as independent criteria of cytoxicity. Cytotoxic effects on human amnion cells were produced by the combination of specific rabbit antibody and factors in normal human serum which were indistinguishable from the components of hemolytic complement and calcium and magnesium ions. The data have been discussed in relation to the apparent multiplicity of serologic systems which can effect cellular injury. The nature of the cell and the source of antibody or normal serum constituents have been emphasized as factors which may influence the experimental selection of a given humoral mechanism.
Article|
December 01 1960
STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT
Ann Ross,
Ann Ross
From the Institute of Pathology, Western Reserve University, Cleveland
Search for other works by this author on:
Irwin H. Lepow
Irwin H. Lepow
From the Institute of Pathology, Western Reserve University, Cleveland
Search for other works by this author on:
Ann Ross
From the Institute of Pathology, Western Reserve University, Cleveland
Irwin H. Lepow
From the Institute of Pathology, Western Reserve University, Cleveland
Received:
July 18 1960
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1960, by The Rockefeller Institute
1960
J Exp Med (1960) 112 (6): 1085–1106.
Article history
Received:
July 18 1960
Citation
Ann Ross, Irwin H. Lepow; STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT . J Exp Med 1 December 1960; 112 (6): 1085–1106. doi: https://doi.org/10.1084/jem.112.6.1085
Download citation file: