Immunization of CBA and C57 mice with a branched, multichain synthetic polypeptide, poly (tyr,glu)-poly DL-ala--poly lys, ((T,G)-A--L), in Freund's complete adjuvant results in a tenfold or more difference in the antigen-binding capacity of sera from the two strains, although they respond equally to bovine serum albumin. Immunization of CBA x C57 F1, F1 x CBA, and F1 x C57 mice reveals definite genetic control of the response to (T,G)-A--L, which appears to be due to a single major genetic factor, with perhaps one or more modifying factors. Immunization of CBA and C57 mice with (H,G)-A--L, a synthetic polypeptide in which histidine replaces tyrosine, gives the opposite result, CBA's respond and C57's do not. From this, it appears that the genetic control of the response to (T,G)-A--L is specific for the antigenic determinant. The implications of these results are discussed.
Article|
September 01 1965
GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE
Hugh O. McDevitt,
Hugh O. McDevitt
From the National Institute for Medical Research, Mill Hill, London, England, and the Weizmann Institute of Science, Rehovoth, Israel
Search for other works by this author on:
Michael Sela
Michael Sela
From the National Institute for Medical Research, Mill Hill, London, England, and the Weizmann Institute of Science, Rehovoth, Israel
Search for other works by this author on:
Hugh O. McDevitt
From the National Institute for Medical Research, Mill Hill, London, England, and the Weizmann Institute of Science, Rehovoth, Israel
Michael Sela
From the National Institute for Medical Research, Mill Hill, London, England, and the Weizmann Institute of Science, Rehovoth, Israel
Received:
April 30 1965
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1965 by The Rockefeller University Press
1965
J Exp Med (1965) 122 (3): 517–531.
Article history
Received:
April 30 1965
Citation
Hugh O. McDevitt, Michael Sela; GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE . J Exp Med 1 September 1965; 122 (3): 517–531. doi: https://doi.org/10.1084/jem.122.3.517
Download citation file: