The recovery of hapten-binding activity by a mixture of H and L polypeptide chains of the whole γG-immunoglobulin fraction from rabbit anti-p-azobenzenearsonate (Rp) serum is almost as great as that by a mixture of H and L chains from specifically purified Rp antibody. Random combination among the H and L chains from the anti-Rp antibodies and the normal γG-immunoglobulin present would result in little recovery of hapten-binding activity. This suggests a preferential recombination of H and L chains from antibody. Mixtures of H or L chains from anti-p-azobenzoate (Xp) antibody and the complementary chains from antibody-depleted γG-immunoglobulin show little hapten-binding. When anti-Xp antibody H chains are added to mixtures of one equivalent of anti-Xp L chain and increasing amounts of non-specific L chain, the hapten-binding by the mixtures decreases, but not as much as if the H chains combined with the L chains randomly. Hapten was not present during these recombination procedures. These data indicate that in the cases of anti-Xp and of anti-Rp antibodies, there is a selective combination between those H and L chains which give effective hapten binding regions.
Article|
October 01 1965
PREFERENTIAL RECOMBINATION OF ANTIBODY CHAINS TO FORM EFFECTIVE BINDING SITES
O. A. Roholt,
O. A. Roholt
From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York
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G. Radzimski,
G. Radzimski
From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York
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D. Pressman
D. Pressman
From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York
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O. A. Roholt
From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York
G. Radzimski
From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York
D. Pressman
From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York
Received:
May 13 1965
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1965 by The Rockefeller University Press
1965
J Exp Med (1965) 122 (4): 785–798.
Article history
Received:
May 13 1965
Citation
O. A. Roholt, G. Radzimski, D. Pressman; PREFERENTIAL RECOMBINATION OF ANTIBODY CHAINS TO FORM EFFECTIVE BINDING SITES . J Exp Med 1 October 1965; 122 (4): 785–798. doi: https://doi.org/10.1084/jem.122.4.785
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