Cytophilic activity for macrophages was shown to be a property possessed by most, if not all, of the complement binding 7S γ2-population of guinea pig antibodies. Cytophilic antibodies were also demonstrated in rabbit and mouse antisera to sheep red cells. While each species antibody best sensitized homologous macrophages, cross species sensitization was also observed. The binding site for macrophages resides on the Fc fragment, therefore, on the H chains, and is destroyed by pepsin hydrolysis or reduction and alkylation. The binding reaction is reversible with a high rate of dissociation at 37°C. Cytophilic activity is not complement dependent, and was shown to be that property of opsonizing antibody which provides the receptors that permit the binding of the antibody to the macrophage cell membrane in preparation for phagocytosis.
Article|
January 01 1966
PROPERTIES OF ANTIBODIES CYTOPHILIC FOR MACROPHAGES
Arthur Berken,
Arthur Berken
From the Department of Pathology, New York University School of Medicine, New York
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Baruj Benacerraf
Baruj Benacerraf
From the Department of Pathology, New York University School of Medicine, New York
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Arthur Berken
From the Department of Pathology, New York University School of Medicine, New York
Baruj Benacerraf
From the Department of Pathology, New York University School of Medicine, New York
Received:
July 07 1965
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1966 by The Rockefeller University Press
1966
J Exp Med (1966) 123 (1): 119–144.
Article history
Received:
July 07 1965
Citation
Arthur Berken, Baruj Benacerraf; PROPERTIES OF ANTIBODIES CYTOPHILIC FOR MACROPHAGES . J Exp Med 1 January 1966; 123 (1): 119–144. doi: https://doi.org/10.1084/jem.123.1.119
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