If viremic sera from mice chronically infected with lactic dehydrogenase virus (LDV) were first treated with ether or ultraviolet light to inactivate the infectious virus, neutralizing antibody could be demonstrated. Significant amounts of antibody, however, were not detected until the mice had been infected for about 2½ months and its presence did not result in the elimination of the chronic viremia. Virus isolated from sera containing neutralizing antibody was found to be relatively resistant to neutralization by anti-LDV. Further studies revealed that the resistant virus existed in the form of an infectious virus-antibody complex (sensitized virus). The presence of such a complex was demonstrated by the fact that the virus fraction which persisted after in vivo or in vitro exposure to mouse anti-LDV was readily neutralized by goat anti-mouse sera or goat anti-mouse γ-globulin, whereas virus that had not been previously exposed to mouse anti-LDV was completely resistant to neutralization by goat anti-mouse sera. These findings suggest that (a) sensitization may play an important role in the resistance and susceptibility of a virus to neutralization by antiviral antibody, and (b) an anti-γ-globulin may prove useful in neutralizing the resistant fraction and in demonstrating otherwise undetectable antiviral antibody.
Article|
July 01 1966
INFECTIOUS VIRUS-ANTIBODY COMPLEX IN THE BLOOD OF CHRONICALLY INFECTED MICE
Abner Louis Notkins,
Abner Louis Notkins
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
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Suellen Mahar,
Suellen Mahar
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
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Christina Scheele,
Christina Scheele
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
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Joel Goffman
Joel Goffman
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
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Abner Louis Notkins
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
Suellen Mahar
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
Christina Scheele
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
Joel Goffman
From the Laboratory of Microbiology, National Institute of Dental Research, United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland
Received:
March 13 1966
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1966 by The Rockefeller University Press
1966
J Exp Med (1966) 124 (1): 81–97.
Article history
Received:
March 13 1966
Citation
Abner Louis Notkins, Suellen Mahar, Christina Scheele, Joel Goffman; INFECTIOUS VIRUS-ANTIBODY COMPLEX IN THE BLOOD OF CHRONICALLY INFECTED MICE . J Exp Med 1 July 1966; 124 (1): 81–97. doi: https://doi.org/10.1084/jem.124.1.81
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