Electron microscopic studies demonstrated that lesions were produced on the endotoxic lipopolysaccharide (LPS) as well as on the cell surface of V. alcalescens after reaction with fresh guinea pig serum. These lesions were approximately 90 A in diameter, and were seen on two characteristic structural entities derived from LPS preparations after incubation with serum. The use of numerous inhibitors, inactivators, and reaction conditions affecting hemolytic C' activity revealed that these lesions were mediated by the C' system. Concomitant with lesion formation, C' was fixed; the effect on classical C'3 activity was pronounced. It is concluded that endotoxic LPS, as contained in the outer three-layered membrane of the bacterial cell, is a substrate for the C' enzymes. It is suggested that certain biological activities of endotoxin may derive from its effects on the C' system.
Article|
May 01 1967
INTERACTIONS OF THE COMPLEMENT SYSTEM WITH THE SURFACE AND ENDOTOXIC LIPOPOLYSACCHARIDE OF VEILLONELLA ALCALESCENS
Howard A. Bladen,
Howard A. Bladen
From the Laboratory of Histology and Pathology and the Immunology Section of the Laboratory of Microbiology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland
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Henry Gewurz,
Henry Gewurz
From the Laboratory of Histology and Pathology and the Immunology Section of the Laboratory of Microbiology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland
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Stephan E. Mergenhagen
Stephan E. Mergenhagen
From the Laboratory of Histology and Pathology and the Immunology Section of the Laboratory of Microbiology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland
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Howard A. Bladen
From the Laboratory of Histology and Pathology and the Immunology Section of the Laboratory of Microbiology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland
Henry Gewurz
From the Laboratory of Histology and Pathology and the Immunology Section of the Laboratory of Microbiology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland
Stephan E. Mergenhagen
From the Laboratory of Histology and Pathology and the Immunology Section of the Laboratory of Microbiology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland
Received:
December 27 1966
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1967 by The Rockefeller University Press
1967
J Exp Med (1967) 125 (5): 767–786.
Article history
Received:
December 27 1966
Citation
Howard A. Bladen, Henry Gewurz, Stephan E. Mergenhagen; INTERACTIONS OF THE COMPLEMENT SYSTEM WITH THE SURFACE AND ENDOTOXIC LIPOPOLYSACCHARIDE OF VEILLONELLA ALCALESCENS . J Exp Med 1 May 1967; 125 (5): 767–786. doi: https://doi.org/10.1084/jem.125.5.767
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