It was shown in the preceding paper that incubation of the rabbit polymorphonuclearleukocyteswith phosphonate esters leads to an irreversible inhibition of the ability of the leukocyte to respond to the chemotactic factor. This "cell-dependent inhibition" was attributed to the inactivation by the phosphonates of an esterase existing in or on the leukocyte in an already activated state. As shown in this paper, incubating the leukocyte with phosphonate in the presence of certain esters prevents this cell-dependent inhibition. The protection is specific; the ester must be an acetate. Ethyl formate, ethyl propionate, ethyl butyrate, glucose 6-phosphate, fructose 1,6-diphosphate, ATP, tosyl arginine methyl ester, or acetyl tyrosine ethyl ester do not protect. The protection is independent of the phosphonate used to inhibit, and the degree of protection depends on the relative concentrations of acetate and phosphonate. Those acetates which protect are also the esters which inhibit chemotaxis when added to the leukocyte in the upper part of the chemotaxis chamber. It is concluded that the activated esterase is an enzyme capable of specifically splitting, or binding acetates, or doing both. Presumably the esterase is some type of acetylesterase or acetylase. The known aliesterase present in the leukocyte is not the activated esterase. Inhibition of the activated esterase by phosphonates has no effect on endogenous or exogenous glycolysis.
Article|
June 01 1967
PARTIAL BIOCHEMICAL CHARACTERIZATION OF THE ACTIVATED ESTERASE REQUIRED IN THE COMPLEMENT-DEPENDENT CHEMOTAXIS OF RABBIT POLYMORPHONUCLEAR LEUKOCYTES
Elmer L. Becker,
Elmer L. Becker
From the Department of Immunochemistry, Walter Reed Army Institute of Research and the Immunobiology Branch, Armed Forces Institute of Pathology, Washington, D. C.
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Peter A. Ward
Peter A. Ward
From the Department of Immunochemistry, Walter Reed Army Institute of Research and the Immunobiology Branch, Armed Forces Institute of Pathology, Washington, D. C.
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Elmer L. Becker
From the Department of Immunochemistry, Walter Reed Army Institute of Research and the Immunobiology Branch, Armed Forces Institute of Pathology, Washington, D. C.
Peter A. Ward
From the Department of Immunochemistry, Walter Reed Army Institute of Research and the Immunobiology Branch, Armed Forces Institute of Pathology, Washington, D. C.
Received:
January 22 1967
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1967 by The Rockefeller University Press
1967
J Exp Med (1967) 125 (6): 1021–1030.
Article history
Received:
January 22 1967
Citation
Elmer L. Becker, Peter A. Ward; PARTIAL BIOCHEMICAL CHARACTERIZATION OF THE ACTIVATED ESTERASE REQUIRED IN THE COMPLEMENT-DEPENDENT CHEMOTAXIS OF RABBIT POLYMORPHONUCLEAR LEUKOCYTES . J Exp Med 1 June 1967; 125 (6): 1021–1030. doi: https://doi.org/10.1084/jem.125.6.1021
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