Two series of experiments were performed, utilizing a modification of the hemolysin plaque technique which registers 19S antibody, in an attempt to determine the frequency of cells capable of simultaneously producing antibody to two non-cross-reacting antigens. Mice were immunized i.v. with rabbit and camel RBC and their spleens assayed for cells producing antibody against both antigens. 16,904 cells producing antibody of one or the other specificity, from 26 mice, were counted. Not one cell was detected which produced antibody of two specificities. Rabbits were immunized intradermally with HSA to which polyalanyl and p-azobenzenearsonate groups were chemically attached. The individual haptens, polyalanyl, and p-azobenzenearsonate groups were coupled to separate aliquots of SRBC, and the lymph nodes of immunized rabbits were assayed for cells releasing antibody against both haptens. In a study of 11 rabbits, after counting 27,845 cells producing antibody, we detected no "double" plaques.
Article|
July 01 1968
STUDIES ON THE COMPETENCE OF SINGLE CELLS TO PRODUCE ANTIBODIES OF TWO SPECIFICITIES
Harriet Gershon,
Harriet Gershon
From The Weizmann Institute of Science, Rehovoth, Israel
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Sara Bauminger,
Sara Bauminger
From The Weizmann Institute of Science, Rehovoth, Israel
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Michael Sela,
Michael Sela
From The Weizmann Institute of Science, Rehovoth, Israel
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Michael Feldman
Michael Feldman
From The Weizmann Institute of Science, Rehovoth, Israel
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Harriet Gershon
From The Weizmann Institute of Science, Rehovoth, Israel
Sara Bauminger
From The Weizmann Institute of Science, Rehovoth, Israel
Michael Sela
From The Weizmann Institute of Science, Rehovoth, Israel
Michael Feldman
From The Weizmann Institute of Science, Rehovoth, Israel
Received:
March 26 1968
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1968 by The Rockefeller University Press
1968
J Exp Med (1968) 128 (1): 223–233.
Article history
Received:
March 26 1968
Citation
Harriet Gershon, Sara Bauminger, Michael Sela, Michael Feldman; STUDIES ON THE COMPETENCE OF SINGLE CELLS TO PRODUCE ANTIBODIES OF TWO SPECIFICITIES . J Exp Med 1 July 1968; 128 (1): 223–233. doi: https://doi.org/10.1084/jem.128.1.223
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