Human antibodies to blood group A substance were purified by absorption on columns of insoluble polyleucyl hog blood group A + H substance and eluted first with N-acetylgalactosamine and then with an A active reduced pentasaccharide ARL0.52. The γM and γG antibodies in these eluates were separated by density gradient centrifugation. The antibodies were studied for their relative capacities to be inhibited by various blood group A active oligosaccharides. Antibodies eluted by the N-acetylgalactosamine could be inhibited by N-acetylgalactosamine, as well as by lower concentrations of A active tri- and pentasaccharides, while those eluted by the pentasaccharide ARL0.52 could only be inhibited by the two oligosaccharides, but not by N-acetylgalactosamine, indicating that the N-acetylgalactosamine eluate had more antibodies with smaller size combining sites than the ARL0.52 eluate. Measurements by equilibrium dialysis gave values ranging from 2 x 103 to 1 x 105 M–1 and the values obtained with the ARL0.52 eluate were somewhat higher than those with the GalNAc eluate. Only one of three anti-A sera had γM anti-A in the ARL0.52 eluate, while all three had γM in the N-acetylgalactosamine eluate. Data on the precipitating, hemagglutinating, complement fixing, hemolytic properties of the eluted antibodies, and of their content of κ and λ light chains are given.
Article|
May 01 1969
STUDIES ON HUMAN ANTIBODIES : VIII. PROPERTIES AND ASSOCIATION CONSTANTS OF HUMAN ANTIBODIES TO BLOOD GROUP A SUBSTANCE PURIFIED WITH INSOLUBLE SPECIFIC ADSORBENTS AND FRACTIONALLY ELUTED WITH MONO- AND OLIGOSACCHARIDE
Carlos Moreno,
Carlos Moreno
From the Departments of Microbiology and Neurology, College of Physicians and Surgeons, Columbia University and the Neurological Institute, Presbyterian Hospital, New York 10032.
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Elvin A. Kabat
Elvin A. Kabat
From the Departments of Microbiology and Neurology, College of Physicians and Surgeons, Columbia University and the Neurological Institute, Presbyterian Hospital, New York 10032.
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Carlos Moreno
From the Departments of Microbiology and Neurology, College of Physicians and Surgeons, Columbia University and the Neurological Institute, Presbyterian Hospital, New York 10032.
Elvin A. Kabat
From the Departments of Microbiology and Neurology, College of Physicians and Surgeons, Columbia University and the Neurological Institute, Presbyterian Hospital, New York 10032.
Received:
December 16 1968
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1969 by The Rockefeller University Press
1969
J Exp Med (1969) 129 (5): 871–896.
Article history
Received:
December 16 1968
Citation
Carlos Moreno, Elvin A. Kabat; STUDIES ON HUMAN ANTIBODIES : VIII. PROPERTIES AND ASSOCIATION CONSTANTS OF HUMAN ANTIBODIES TO BLOOD GROUP A SUBSTANCE PURIFIED WITH INSOLUBLE SPECIFIC ADSORBENTS AND FRACTIONALLY ELUTED WITH MONO- AND OLIGOSACCHARIDE . J Exp Med 1 May 1969; 129 (5): 871–896. doi: https://doi.org/10.1084/jem.129.5.871
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