In this study, the frequency of uropod formation and the type of lymphocyte bearing the uropod was investigated in various guinea pig lymphocyte populations. Without prior in vitro stimulation, almost 40% of peritoneal exudate lymphocytes (PELS) form uropods, while thymocytes and lymph node cells form far fewer. Subsequent stimulation in vitro with purified protein derivative demonstrated that there is an association between antigen reactivity and frequency of uropod formation in these populations. The ultrastructure of these uropods is identical to that described for human lymphocytes stimulated with phytohemagglutinin. In the populations studied, all the lymphocytes forming uropods lack easily detectable surface membrane immunoglobulin and are therefore most likely thymus-derived or T lymphocytes.
Article|
May 01 1972
THE UROPOD-BEARING LYMPHOCYTE OF THE GUINEA PIG : EVIDENCE FOR THYMIC ORIGIN
David L. Rosenstreich,
David L. Rosenstreich
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
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Ethan Shevach,
Ethan Shevach
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
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Ira Green,
Ira Green
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
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Alan S. Rosenthal
Alan S. Rosenthal
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
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David L. Rosenstreich
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Ethan Shevach
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Ira Green
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Alan S. Rosenthal
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Received:
December 29 1971
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1972 by The Rockefeller University Press
1972
J Exp Med (1972) 135 (5): 1037–1048.
Article history
Received:
December 29 1971
Citation
David L. Rosenstreich, Ethan Shevach, Ira Green, Alan S. Rosenthal; THE UROPOD-BEARING LYMPHOCYTE OF THE GUINEA PIG : EVIDENCE FOR THYMIC ORIGIN . J Exp Med 1 May 1972; 135 (5): 1037–1048. doi: https://doi.org/10.1084/jem.135.5.1037
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