A higher percentage of specific antigen-binding cells can be detected not only in normal CBA/J mouse spleen cell preparations (0.99%), but also in the normal thymus cell preparations (0.15%) with the use of [125I]2,4-dinitrophenyl-human IgG (DNP-HGG) as compared with most other antigens employed under similar conditions. The receptors on these cells are mainly specific for the DNP group as shown by the inhibition studies with DNP-lysine and the other DNP conjugates. In addition, it was shown by the inhibition studies with DNP-lysine that the thymus cells seem to have a lower avidity for DNP than the spleen cells. Preincubation of cell suspensions with antisera to immunoglobulins showed that the DNP-HGG antigen-binding cells in the thymus are inhibited predominantly with anti-µ-chain serum and the spleen cells with both anti-µ-chain and anti-γ-chain sera; both cell populations were also significantly inhibited with the antisera to κ-chains and Fab fragments. These data indicate that the nature of the receptor on the T cell differs from that on the majority of spleen cells.
Article|
February 01 1973
2,4-DINITROPHENYL RECEPTORS ON MOUSE THYMUS AND SPLEEN CELLS
David A. Lawrence,
David A. Lawrence
From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
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Hans L. Spiegelberg,
Hans L. Spiegelberg
From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
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William O. Weigle
William O. Weigle
From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
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David A. Lawrence
From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
Hans L. Spiegelberg
From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
William O. Weigle
From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037
Received:
September 22 1972
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 137 (2): 470–482.
Article history
Received:
September 22 1972
Citation
David A. Lawrence, Hans L. Spiegelberg, William O. Weigle; 2,4-DINITROPHENYL RECEPTORS ON MOUSE THYMUS AND SPLEEN CELLS . J Exp Med 1 February 1973; 137 (2): 470–482. doi: https://doi.org/10.1084/jem.137.2.470
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