Antibody to herpes simplex virus (HSV) plus complement destroyed HSV-infected cells but did not stop the spread of the infection. Studies on the relationship between the time of appearance of viral antigens on the cell surface, immunological destruction of the cells by antiviral antibody and complement, and transfer of the virus to adjacent cells showed that the virus spread from infected to uninfected cells before the infected cells were susceptible to immunological destruction. Incubation of infected monolayers with leukocytes, however, stopped the spread of the virus by nonspecifically damaging both infected and uninfected cells and by presumably breaking intercellular bridges. When leukocytes were removed from infected monolayers, viral plaques developed. If, however, antiviral antibody and complement were added to monolayers before the leukocytes were removed, the development of plaques was prevented. These findings suggest that both antibody and leukocytes are needed to cure HSV infections.
Article|
March 01 1973
PREVENTION OF CELL-TO-CELL SPREAD OF HERPES SIMPLEX VIRUS BY LEUKOCYTES
Donald Louis Lodmell,
Donald Louis Lodmell
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
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Akira Niwa,
Akira Niwa
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
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Kozaburo Hayashi,
Kozaburo Hayashi
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
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Abner Louis Notkins
Abner Louis Notkins
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
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Donald Louis Lodmell
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
Akira Niwa
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
Kozaburo Hayashi
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
Abner Louis Notkins
From the Laboratory of Microbiology and Immunology, Virology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
Received:
October 20 1972
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 137 (3): 706–720.
Article history
Received:
October 20 1972
Citation
Donald Louis Lodmell, Akira Niwa, Kozaburo Hayashi, Abner Louis Notkins; PREVENTION OF CELL-TO-CELL SPREAD OF HERPES SIMPLEX VIRUS BY LEUKOCYTES . J Exp Med 1 March 1973; 137 (3): 706–720. doi: https://doi.org/10.1084/jem.137.3.706
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