Neuraminidase treatment of normal human lymphocytes enhances their capacity to form SRBC rosettes; more red cells are bound and the rosettes are more stable. Under these conditions approximately 90% of peripheral blood lymphocytes form rosettes. In addition to the effects on T cells, another population of lymphocytes which possess surface immunoglobulin and have the Fc receptor acquire the rosette-forming property after neuraminidase. This subpopulation of ‘B’ cells represents approximately half of the lymphocytes with surface immunoglobulin but is not found among the leukemic lymphocytes of patients with chronic lymphocytic leukemia. Electron microscope observations indicate close approximation and intimate association of the red cell and lymphocyte membranes after neuraminidase.
Brief Definitive Report|
June 01 1973
SHEEP RED CELL BINDING TO HUMAN LYMPHOCYTES TREATED WITH NEURAMINIDASE; ENHANCEMENT OF T CELL BINDING AND IDENTIFICATION OF A SUBPOPULATION OF B CELLS
Z. Bentwich,
Z. Bentwich
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
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S. D. Douglas,
S. D. Douglas
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
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E. Skutelsky,
E. Skutelsky
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
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H. G. Kunkel
H. G. Kunkel
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
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Z. Bentwich
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
S. D. Douglas
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
E. Skutelsky
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
H. G. Kunkel
From The Rockefeller University, New York 10021 and the Department of Medicine, Mount Sinai School of Medicine of the City University of New York, New York 10016
Received:
April 03 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 137 (6): 1532–1537.
Article history
Received:
April 03 1973
Citation
Z. Bentwich, S. D. Douglas, E. Skutelsky, H. G. Kunkel; SHEEP RED CELL BINDING TO HUMAN LYMPHOCYTES TREATED WITH NEURAMINIDASE; ENHANCEMENT OF T CELL BINDING AND IDENTIFICATION OF A SUBPOPULATION OF B CELLS . J Exp Med 1 June 1973; 137 (6): 1532–1537. doi: https://doi.org/10.1084/jem.137.6.1532
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