Cutaneous basophil hypersensitivity, an immune inflammatory reaction characterized by infiltrates of basophils and a delayed time-course, was studied in guinea pigs contact sensitized with oxazolone. Routine histological techniques, employing ordinary paraffin sections, were modified to study this reaction. When biopsies of contact lesions were processed by these methods dense infiltrates of basophils could be demonstrated. Animals sensitized with complete Freund's adjuvant emulsified with oxazolone-keyhole limpet hemocyanin conjugates also developed delayed-in-time responses to contact challenge with oxazolone but not to picryl chloride. These hapten-specific delayed-in-time reactions also contained substantial numbers of basophils. Transfer of serum from actively sensitized guinea pigs resulted in specific accumulation of basophils at challenge sites of recipients. Thus, in this experimental system, cutaneous basophil hypersensitivity was found to be a hapten-specific delayed-in-time reaction that could be transferred with immune serum.
Article|
November 01 1973
CUTANEOUS BASOPHIL HYPERSENSITIVITY IN CONTACT-SENSITIZED GUINEA PIGS : I. TRANSFER WITH IMMUNE SERUM
P. W. Askenase
P. W. Askenase
From the Department of Medicine, Yak University School of Medicine, New Haven, Connecticut 06510
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P. W. Askenase
From the Department of Medicine, Yak University School of Medicine, New Haven, Connecticut 06510
Received:
June 13 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 138 (5): 1144–1155.
Article history
Received:
June 13 1973
Citation
P. W. Askenase; CUTANEOUS BASOPHIL HYPERSENSITIVITY IN CONTACT-SENSITIZED GUINEA PIGS : I. TRANSFER WITH IMMUNE SERUM . J Exp Med 1 November 1973; 138 (5): 1144–1155. doi: https://doi.org/10.1084/jem.138.5.1144
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