Antigen activation of DNA synthesis in immune thymus-derived lymphocytes of guinea pigs requires the cooperation of macrophages and lymphocytes. We have investigated the role of histocompatibility determinants in this macrophage-lymphocyte interaction using cells from inbred strain 2 and 13 guinea pigs. The data demonstrate that efficient presentation of macrophage-associated antigen to the lymphocyte requires identity between macrophage and lymphocyte at some portion of the major histocompatibility complex. The failure of allogeneic macrophages to effectively initiate immune lymphocyte proliferation was not the result of the presence of an inhibitor of blastogenesis released in mixtures of allogeneic cells, peculiarities of the antigen or lymphoid cells employed, nor differing kinetics of activation by allogeneic macrophages. In addition, data were presented that demonstrated that alloantisera inhibit lymphocyte DNA synthesis by functional interference with macrophage-lymphocyte interaction.
Article|
November 01 1973
FUNCTION OF MACROPHAGES IN ANTIGEN RECOGNITION BY GUINEA PIG T LYMPHOCYTES : I. REQUIREMENT FOR HISTOCOMPATIBLE MACROPHAGES AND LYMPHOCYTES
Alan S. Rosenthal,
Alan S. Rosenthal
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
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Ethan M. Shevach
Ethan M. Shevach
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
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Alan S. Rosenthal
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Ethan M. Shevach
From the Laboratory of Clinical Investigation and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Received:
July 12 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 138 (5): 1194–1212.
Article history
Received:
July 12 1973
Citation
Alan S. Rosenthal, Ethan M. Shevach; FUNCTION OF MACROPHAGES IN ANTIGEN RECOGNITION BY GUINEA PIG T LYMPHOCYTES : I. REQUIREMENT FOR HISTOCOMPATIBLE MACROPHAGES AND LYMPHOCYTES . J Exp Med 1 November 1973; 138 (5): 1194–1212. doi: https://doi.org/10.1084/jem.138.5.1194
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