Unprimed mouse spleen cells cultured in vitro on syngeneic tumor cell monolayers have been previously shown to become specifically sensitized and to mediate cytotoxicity against the same type of tumor cells. This complete in vitro system of cell-mediated response has been presently used to test the effect of a thymic humoral factor (THF) upon the differentiation process leading to the generation of specifically committed lymphocytes. Culture media were supplemented with 2% THF during either the sensitization or effector phase, or both phases of the reaction. Whereas the addition of THF during both phases or during sensitization only resulted in a significant increase in the cytotoxicity index, THF added during the effector phase was ineffective. The behavior of unsensitized spleen cells and of spleen cells sensitized against nonrelated transplantation antigens remained unmodified by THF. After showing that the entire reaction is mediated by lymphocytes of thymic origin, THF was directly tested on T or B spleen cells. It was found that only T cells reacted to THF by an increased cytotoxic capacity, while B cells remained inactive after addition of THF. It was therefore concluded that THF activates a postthymic population of lymphoid cells, transforming them into fully competent lymphocytes.
Article|
December 01 1973
INCREASED REACTIVITY OF MOUSE SPLEEN CELLS SENSITIZED IN VITRO AGAINST SYNGENEIC TUMOR CELLS IN THE PRESENCE OF A THYMIC HUMORAL FACTOR
Claude Carnaud,
Claude Carnaud
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
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David Ilfeld,
David Ilfeld
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
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Itzhak Brook,
Itzhak Brook
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
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Nathan Trainin
Nathan Trainin
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
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Claude Carnaud
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
David Ilfeld
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Itzhak Brook
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Nathan Trainin
From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Received:
June 28 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1973 by The Rockefeller University Press
1973
J Exp Med (1973) 138 (6): 1521–1532.
Article history
Received:
June 28 1973
Citation
Claude Carnaud, David Ilfeld, Itzhak Brook, Nathan Trainin; INCREASED REACTIVITY OF MOUSE SPLEEN CELLS SENSITIZED IN VITRO AGAINST SYNGENEIC TUMOR CELLS IN THE PRESENCE OF A THYMIC HUMORAL FACTOR . J Exp Med 1 December 1973; 138 (6): 1521–1532. doi: https://doi.org/10.1084/jem.138.6.1521
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