Mice treated with sea star factor (SSF), a protein extracted from sea star coelomocytes, became highly susceptible to infection with a normally sublethal dose of Listeria monocytogenes. This was in contrast to the expected result of increased resistance originally postulated because of the macrophage-activating properties of SSF. Enhanced susceptibility was seen when SSF was given from 96 h before to 48 h after infection with Listeria. Mice pretreated with SSF failed to develop immunity to Listeria when given a dose of organisms capable of immunizing nontreated mice. Treatment of immune mice with SSF did not alter their immune status. In addition, incubation of immune lymphocytes with SSF in vitro did not alter their ability to adoptively transfer immunity to normal recipients. Immune lymphocytes treated with SSF and then incubated with anti-SSF and C did, however, lose the ability to transfer immunity. These results suggest that SSF enhances infection by binding to T lymphocytes, inhibiting their replication upon contact with Listeria antigen and thus preventing the generation of a population of sensitized lymphocytes capable of effecting anti-Listeria immunity.
Article|
April 01 1974
THE EFFECT OF SEA STAR COELOMOCYTE EXTRACT ON CELL-MEDIATED RESISTANCE TO LISTERIA MONOCYTOGENES IN MICE
David O. Willenborg,
David O. Willenborg
From the Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Search for other works by this author on:
Robert A. Prendergast
Robert A. Prendergast
From the Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Search for other works by this author on:
David O. Willenborg
From the Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Robert A. Prendergast
From the Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Received:
November 28 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1974 by The Rockefeller University Press
1974
J Exp Med (1974) 139 (4): 820–833.
Article history
Received:
November 28 1973
Citation
David O. Willenborg, Robert A. Prendergast; THE EFFECT OF SEA STAR COELOMOCYTE EXTRACT ON CELL-MEDIATED RESISTANCE TO LISTERIA MONOCYTOGENES IN MICE . J Exp Med 1 April 1974; 139 (4): 820–833. doi: https://doi.org/10.1084/jem.139.4.820
Download citation file: