The present studies have shown that two subpopulations of thymus-dependent lymphocytes may act synergistically in the mixed lymphocyte reaction (MLR) in the mouse. One subpopulation was well represented in the young adult thymus and the other in lymph nodes. For optimum synergy, both populations must be allogeneic to the stimulator cells. Pretreatment of either population with mitomycin-C abolished synergy. Anti-θ serum abolished both MLR responding and synergizing activities of lymphoid cells. The two thymus-dependent subpopulations were both present in the spleen, and displayed different migratory patterns when injected into irradiated mice: one population went to spleens of the irradiated mice, the other to lymph nodes. The effects of anti-thymocyte serum on the MLR and upon synergy were assessed. While minor differences exist and are herein described, our overall results strongly suggest that in our experiments with synergy in MLR, we may be dealing with the same T1- and T2-cell subpopulations described by Cantor and Asofsky and coworkers (1, 2, 4, 5, 14) as displaying synergy in the graft-vs.-host reaction.
Article|
June 01 1974
SYNERGY AMONG RESPONDING LYMPHOID CELLS IN THE ONE-WAY MIXED LYMPHOCYTE REACTION : INTERACTION BETWEEN TWO TYPES OF THYMUS-DEPENDENT CELLS
Wolfgang Tittor,
Wolfgang Tittor
From the Department of Pathology, UCLA School of Medicine, Los Angles, California 90024
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Maria Gerbase-Delima,
Maria Gerbase-Delima
From the Department of Pathology, UCLA School of Medicine, Los Angles, California 90024
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Roy L. Walford
Roy L. Walford
From the Department of Pathology, UCLA School of Medicine, Los Angles, California 90024
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Wolfgang Tittor
From the Department of Pathology, UCLA School of Medicine, Los Angles, California 90024
Maria Gerbase-Delima
From the Department of Pathology, UCLA School of Medicine, Los Angles, California 90024
Roy L. Walford
From the Department of Pathology, UCLA School of Medicine, Los Angles, California 90024
Received:
December 17 1973
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1974 by The Rockefeller University Press
1974
J Exp Med (1974) 139 (6): 1488–1498.
Article history
Received:
December 17 1973
Citation
Wolfgang Tittor, Maria Gerbase-Delima, Roy L. Walford; SYNERGY AMONG RESPONDING LYMPHOID CELLS IN THE ONE-WAY MIXED LYMPHOCYTE REACTION : INTERACTION BETWEEN TWO TYPES OF THYMUS-DEPENDENT CELLS . J Exp Med 1 June 1974; 139 (6): 1488–1498. doi: https://doi.org/10.1084/jem.139.6.1488
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