This study shows that bone marrow-derived lymphocytes of guinea pigs if appropriately activated produce a monocyte chemotactic factor (MNL CTX). Activation of B lymphocytes to produce a chemotactic lymphokine occurs subsequent to interactions with a variety of membrane-associated receptors. Polymeric B-cell mitogens with multiple binding sites, polymerized flagellin and lipopolysaccharide, initiated mediator synthesis. Furthermore, interaction of antigen-antibody complexes or aggregated gamma globulin with the Fc receptor and binding of antigen-antibody-complement complexes at the C3 receptor can effectively facilitate mediator production in the absence of a significant proliferative response. Additionally, intact anti-immunoglobulin but not its Fab fragments activated the B cells. An anti-Fab effectively converted the inactive Fab-bound B cells into producers of MNL CTX, suggesting that the basic mechanism of activation depended upon cross-linking of receptors. Thus, interaction of B-cell surface receptors such as Fc, Ig, and C3 sites with mitogenic as well as nonmitogenic molecules capable of bridging the receptors appears to trigger B-cell mediator production.
Article|
December 01 1974
INDUCTION OF GUINEA PIG B-CELL LYMPHOKINE SYNTHESIS BY MITOGENIC AND NONMITOGENIC SIGNALS TO Fc, Ig, AND C3 RECEPTORS
S. M. Wahl,
S. M. Wahl
From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
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G. M. Iverson,
G. M. Iverson
From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
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J. J. Oppenheim
J. J. Oppenheim
From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
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S. M. Wahl
From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
G. M. Iverson
From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
J. J. Oppenheim
From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014
Received:
July 29 1974
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright © 1974 by The Rockefeller University Press
1974
J Exp Med (1974) 140 (6): 1631–1645.
Article history
Received:
July 29 1974
Citation
S. M. Wahl, G. M. Iverson, J. J. Oppenheim; INDUCTION OF GUINEA PIG B-CELL LYMPHOKINE SYNTHESIS BY MITOGENIC AND NONMITOGENIC SIGNALS TO Fc, Ig, AND C3 RECEPTORS . J Exp Med 1 December 1974; 140 (6): 1631–1645. doi: https://doi.org/10.1084/jem.140.6.1631
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