The ability of T and B lymphocytes to migrate into skin allografts undergoing rejection was studied in mice. Spleen cells from CBA/J mice sensitized to transplantation antigens of A/J or C57BL/6 mice were separated on immunabsorbent columns into purified populations of T and B cells, labeled in vitro with 3H-uridine and injected intravenously into CBA/J mice with 7-day old skin iso and allografts (A/J or C57BL/6). The mice were sacrificed 24 h later and studied by autoradiography. After transfer of either unfractionated spleen cells or T cells, large numbers of labeled cells were found in the cellular infiltrate of allografts, whereas extremely few were seen in isografts. In contrast, after transfer of B cells, almost no labeled cells were detected either in the allografts or the isografts, although they, like T cells, homed normally to lymphoid tissue.
Article|
May 01 1975
T-cell migration into allografts.
A K Bhan
C L Reinisch
R H Levey
R T McCluskey
S F Schlossman
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1975) 141 (5): 1210–1215.
Citation
A K Bhan, C L Reinisch, R H Levey, R T McCluskey, S F Schlossman; T-cell migration into allografts.. J Exp Med 1 May 1975; 141 (5): 1210–1215. doi: https://doi.org/10.1084/jem.141.5.1210
Download citation file: